PMID- 25222227
OWN - NLM
STAT- MEDLINE
DCOM- 20160425
LR  - 20231213
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 13
IP  - 3
DP  - 2014 Sep 5
TI  - Isolation and characterization of CCoAOMT in interspecific hybrid of Acacia 
      auriculiformis x Acacia mangium--a key gene in lignin biosynthesis.
PG  - 7217-38
LID - 10.4238/2014.September.5.7 [doi]
AB  - This study was directed at the understanding of the function of CCoAOMT isolated 
      from Acacia auriculiformis x Acacia mangium. Full length cDNA of the Acacia 
      hybrid CCoAOMT (AhCCoAOMT) was 1024-bp long, containing 750-bp coding regions, 
      with one major open reading frame of 249 amino acids. On the other hand, full 
      length genomic sequence of the CCoAOMT (AhgflCCoAOMT) was 2548 bp long, 
      containing three introns and four exons with a 5' untranslated region (5'UTR) of 
      391 bp in length. The 5'UTR of the characterized CCoAOMT gene contains various 
      regulatory elements. Southern analysis revealed that the Acacia hybrid has more 
      than three copies of the CCoAOMT gene. Real-time PCR showed that this gene was 
      expressed in root, inner bark, leaf, flower and seed pod of the Acacia hybrid. 
      Downregulation of the homologous CCoAOMT gene in tobacco by antisense (AS) and 
      intron-containing hairpin (IHP) constructs containing partial AhCCoAOMT led to 
      reduction in lignin content. Expression of the CCoAOMT in AS line (pART-HAS78-03) 
      and IHP line (pART-HIHP78-06) was reduced respectively by 37 and 75% compared to 
      the control, resulting in a decrease in the estimated lignin content by 24 and 
      56%, respectively. AhCCoAOMT was found to have altered not only S and G units but 
      also total lignin content, which is of economic value to the pulp industry. 
      Subsequent polymorphism analysis of this gene across eight different genetic 
      backgrounds each of A. mangium and A. auriculiformis revealed 47 single 
      nucleotide polymorphisms (SNPs) in A. auriculiformis CCoAOMT and 30 SNPs in A. 
      mangium CCoAOMT.
FAU - Pang, S L
AU  - Pang SL
AD  - Tree Breeding Unit, Applied Forest Science and Industry Development, Sarawak 
      Forestry Corporation, Kuching, Sarawak, Malaysia.
FAU - Ong, S S
AU  - Ong SS
AD  - Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, Kuala 
      Lumpur, Malaysia.
FAU - Lee, H H
AU  - Lee HH
AD  - Department of Biological Science, Faculty of Science, Universiti Tunku Abdul 
      Rahman, Kampar, Perak, Malaysia.
FAU - Zamri, Z
AU  - Zamri Z
AD  - School of Bioscience and Biotechnology, Faculty of Science and Technology, 
      Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
FAU - Kandasamy, K I
AU  - Kandasamy KI
AD  - Malaysia Biotechnology Corporation, Kuala Lumpur, Malaysia.
FAU - Choong, C Y
AU  - Choong CY
AD  - School of Environmental and Natural Resource Sciences, Faculty of Science and 
      Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
FAU - Wickneswari, R
AU  - Wickneswari R
AD  - School of Environmental and Natural Resource Sciences, Faculty of Science and 
      Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia 
      wicki@ukmi.edu.my.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140905
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (DNA, Complementary)
RN  - 9005-53-2 (Lignin)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 2.1.1.104 (caffeoyl-CoA O-methyltransferase)
SB  - IM
MH  - Acacia/*genetics/*metabolism
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics
MH  - Gene Expression
MH  - Gene Expression Regulation, Plant
MH  - Gene Order
MH  - Genetic Vectors/genetics
MH  - *Hybridization, Genetic
MH  - Lignin/*biosynthesis
MH  - Methyltransferases/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Organ Specificity/genetics
MH  - Plant Stems/cytology/genetics
MH  - Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
MH  - Regulatory Elements, Transcriptional
MH  - Sequence Alignment
MH  - Nicotiana/genetics/metabolism
EDAT- 2014/09/16 06:00
MHDA- 2016/04/26 06:00
CRDT- 2014/09/16 06:00
PHST- 2014/09/16 06:00 [entrez]
PHST- 2014/09/16 06:00 [pubmed]
PHST- 2016/04/26 06:00 [medline]
AID - gmr3253 [pii]
AID - 10.4238/2014.September.5.7 [doi]
PST - epublish
SO  - Genet Mol Res. 2014 Sep 5;13(3):7217-38. doi: 10.4238/2014.September.5.7.