PMID- 25222736
OWN - NLM
STAT- MEDLINE
DCOM- 20150528
LR  - 20240314
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 9
DP  - 2014
TI  - The hybrid type polyketide synthase SteelyA is required for cAMP signalling in 
      early Dictyostelium development.
PG  - e106634
LID - 10.1371/journal.pone.0106634 [doi]
LID - e106634
AB  - BACKGROUND: In our previous study we found that the expression of stlA showed 
      peaks both in the early and last stages of development and that a product of 
      SteelyA, 4-methyl-5-pentylbenzene-1,3-diol (MPBD), controlled Dictyostelium spore 
      maturation during the latter. In this study we focused on the role of SteelyA in 
      early stage development. PRINCIPAL FINDINGS: Our stlA null mutant showed 
      aggregation delay and abnormally small aggregation territories. Chemotaxis 
      analysis revealed defective cAMP chemotaxis in the stlA null mutant. cAMP 
      chemotaxis was restored by MPBD addition during early stage development. Assay 
      for cAMP relay response revealed that the stlA null mutant had lower cAMP 
      accumulation during aggregation, suggesting lower ACA activity than the wild type 
      strain. Exogenous cAMP pulses rescued the aggregation defect of the stlA null 
      strain in the absence of MPBD. Expression analysis of cAMP signalling genes 
      revealed lower expression levels in the stlA null mutant during aggregation. 
      CONCLUSION: Our data indicate a regulatory function by SteelyA on cAMP signalling 
      during aggregation and show that SteelyA is indispensable for full activation of 
      ACA.
FAU - Narita, Takaaki B
AU  - Narita TB
AD  - Graduate School of Science and Technology, Sophia University, Tokyo, Japan; 
      Research Fellow of Japan Society for the Promotion of Science (DC2), Tokyo, 
      Japan.
FAU - Chen, Zhi-hui
AU  - Chen ZH
AD  - College of Life Sciences, University of Dundee, Dundee, United Kingdom.
FAU - Schaap, Pauline
AU  - Schaap P
AD  - College of Life Sciences, University of Dundee, Dundee, United Kingdom.
FAU - Saito, Tamao
AU  - Saito T
AD  - Department of Materials and Life Sciences, Sophia University, Tokyo, Japan.
LA  - eng
GR  - 100293/Wellcome Trust/United Kingdom
GR  - BB/G020426/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140915
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Protozoan Proteins)
RN  - 79956-01-7 (Polyketide Synthases)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Chemotaxis/genetics
MH  - Cyclic AMP/*metabolism
MH  - Dictyostelium/genetics/*growth & development/metabolism
MH  - Down-Regulation
MH  - Gene Deletion
MH  - Polyketide Synthases/genetics/metabolism/*physiology
MH  - Protozoan Proteins/genetics/metabolism/*physiology
MH  - Signal Transduction/genetics
PMC - PMC4164351
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/09/16 06:00
MHDA- 2015/05/29 06:00
PMCR- 2014/09/15
CRDT- 2014/09/16 06:00
PHST- 2014/05/23 00:00 [received]
PHST- 2014/08/08 00:00 [accepted]
PHST- 2014/09/16 06:00 [entrez]
PHST- 2014/09/16 06:00 [pubmed]
PHST- 2015/05/29 06:00 [medline]
PHST- 2014/09/15 00:00 [pmc-release]
AID - PONE-D-14-23038 [pii]
AID - 10.1371/journal.pone.0106634 [doi]
PST - epublish
SO  - PLoS One. 2014 Sep 15;9(9):e106634. doi: 10.1371/journal.pone.0106634. 
      eCollection 2014.