PMID- 25223586
OWN - NLM
STAT- MEDLINE
DCOM- 20150514
LR  - 20181202
IS  - 1365-2869 (Electronic)
IS  - 0962-1105 (Linking)
VI  - 24
IP  - 2
DP  - 2015 Apr
TI  - Spontaneous sleep-wake cycle and sleep deprivation differently induce Bdnf1, 
      Bdnf4 and Bdnf9a DNA methylation and transcripts levels in the basal forebrain 
      and frontal cortex in rats.
PG  - 124-30
LID - 10.1111/jsr.12242 [doi]
AB  - Brain-derived neurotrophic factor (Bdnf) regulates neuronal plasticity, slow wave 
      activity and sleep homeostasis. Environmental stimuli control Bdnf expression 
      through epigenetic mechanisms, but there are no data on epigenetic regulation of 
      Bdnf by sleep or sleep deprivation. Here we investigated whether 5-methylcytosine 
      (5mC) DNA modification at Bdnf promoters p1, p4 and p9 influences Bdnf1, Bdnf4 
      and Bdnf9a expression during the normal inactive phase or after sleep deprivation 
      (SD) (3, 6 and 12 h, end-times being ZT3, ZT6 and ZT12) in rats in two brain 
      areas involved in sleep regulation, the basal forebrain and cortex. We found a 
      daytime variation in cortical Bdnf expression: Bdnf1 expression was highest at 
      ZT6 and Bdnf4 lowest at ZT12. Such variation was not observed in the basal 
      forebrain. Also Bdnf p1 and p9 methylation levels differed only in the cortex, 
      while Bdnf p4 methylation did not vary in either area. Factorial analysis 
      revealed that sleep deprivation significantly induced Bdnf1 and Bdnf4 with the 
      similar pattern for Bdnf9a in both basal forebrain and cortex; 12 h of sleep 
      deprivation decreased 5mC levels at the cortical Bdnf p4 and p9. Regression 
      analysis between the 5mC promoter levels and the corresponding Bdnf transcript 
      expression revealed significant negative correlations for the basal forebrain 
      Bdnf1 and cortical Bdnf9a transcripts in only non-deprived rats, while these 
      correlations were lost after sleep deprivation. Our results suggest that Bdnf 
      transcription during the light phase of undisturbed sleep-wake cycle but not 
      after SD is regulated at least partially by brain site-specific DNA methylation.
CI  - (c) 2014 European Sleep Research Society.
FAU - Ventskovska, Olena
AU  - Ventskovska O
AD  - Institute of Biomedicine, University of Helsinki, Helsinki, Finland; Institute 
      for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences 
      of Ukraine, Kharkov, Ukraine.
FAU - Porkka-Heiskanen, Tarja
AU  - Porkka-Heiskanen T
FAU - Karpova, Nina N
AU  - Karpova NN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140915
PL  - England
TA  - J Sleep Res
JT  - Journal of sleep research
JID - 9214441
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Basal Forebrain/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Circadian Rhythm/*genetics
MH  - *DNA Methylation
MH  - Epigenesis, Genetic
MH  - Frontal Lobe/*metabolism
MH  - Male
MH  - Neuronal Plasticity
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Rats
MH  - Sleep/*genetics
MH  - Sleep Deprivation/*genetics/physiopathology
MH  - Time Factors
MH  - Wakefulness/*genetics
OTO - NOTNLM
OT  - Bdnf exon
OT  - circadian
OT  - diurnal
OT  - methylated DNA immunoprecipitation
OT  - sleep homeostasis
EDAT- 2014/09/17 06:00
MHDA- 2015/05/15 06:00
CRDT- 2014/09/17 06:00
PHST- 2014/05/12 00:00 [received]
PHST- 2014/08/10 00:00 [accepted]
PHST- 2014/09/17 06:00 [entrez]
PHST- 2014/09/17 06:00 [pubmed]
PHST- 2015/05/15 06:00 [medline]
AID - 10.1111/jsr.12242 [doi]
PST - ppublish
SO  - J Sleep Res. 2015 Apr;24(2):124-30. doi: 10.1111/jsr.12242. Epub 2014 Sep 15.