PMID- 25223733
OWN - NLM
STAT- MEDLINE
DCOM- 20150128
LR  - 20211021
IS  - 1528-1167 (Electronic)
IS  - 0013-9580 (Print)
IS  - 0013-9580 (Linking)
VI  - 55
IP  - 11
DP  - 2014 Nov
TI  - Effect of spontaneous seizures on GABAA receptor alpha4 subunit expression in an 
      animal model of temporal lobe epilepsy.
PG  - 1826-33
LID - 10.1111/epi.12771 [doi]
AB  - OBJECTIVE: Temporal lobe epilepsy (TLE) is frequently medically intractable and 
      often progressive. Compromised inhibitory neurotransmission due to altered 
      gamma-aminobutyric acid (GABA)A receptor alpha4 subunit (GABAA Ralpha4) expression has been 
      emphasized as a potential contributor to the initial development of epilepsy 
      following a brain insult (primary epileptogenesis), but the regulation of GABAA 
      Ralpha4 during chronic epilepsy, specifically, how expression is altered following 
      spontaneous seizures, is less well understood. METHODS: Continuous 
      video-electroencephalography (EEG) recordings from rats with pilocarpine-induced 
      TLE were used to capture epileptic animals within 3 h of a spontaneous seizure 
      (SS), or >24 h after the last SS, to determine whether recent occurrence of a 
      seizure was associated with altered levels of GABAA Ralpha4 expression. We further 
      evaluated whether this GABAA Ralpha4 plasticity is regulated by signaling mechanisms 
      active in primary epileptogenesis, specifically, increases in brain-derived 
      neurotrophic factor (BDNF) and early growth response factor 3 (Egr3). RESULTS: 
      Elevated levels of GABAA Ralpha4 messenger RNA (mRNA) and protein were observed 
      following spontaneous seizures, and were associated with higher levels of BDNF 
      and Egr3 mRNA. SIGNIFICANCE: These data suggest that spontaneous, recurrent 
      seizures that define chronic epilepsy may influence changes in GABAA Ralpha4 
      expression, and that signaling pathways known to regulate GABAA Ralpha4 expression 
      after status epilepticus may also be activated after spontaneous seizures in 
      chronically epileptic animals.
CI  - Wiley Periodicals, Inc. (c) 2014 International League Against Epilepsy.
FAU - Grabenstatter, Heidi L
AU  - Grabenstatter HL
AD  - Department of Pediatrics, Section of Neurology, Translational Epilepsy Research 
      Program, University of Colorado, AMC, Aurora, Colorado, U.S.A.
FAU - Cogswell, Meaghan
AU  - Cogswell M
FAU - Cruz Del Angel, Yasmin
AU  - Cruz Del Angel Y
FAU - Carlsen, Jessica
AU  - Carlsen J
FAU - Gonzalez, Marco I
AU  - Gonzalez MI
FAU - Raol, Yogendra H
AU  - Raol YH
FAU - Russek, Shelley J
AU  - Russek SJ
FAU - Brooks-Kayal, Amy R
AU  - Brooks-Kayal AR
LA  - eng
GR  - R01 NS051710/NS/NINDS NIH HHS/United States
GR  - R21 NS083057/NS/NINDS NIH HHS/United States
GR  - R01NS051710/NS/NINDS NIH HHS/United States
GR  - P30 NS048154/NS/NINDS NIH HHS/United States
GR  - T32 GM008541/GM/NIGMS NIH HHS/United States
GR  - UL1 TR001082/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140915
PL  - United States
TA  - Epilepsia
JT  - Epilepsia
JID - 2983306R
RN  - 0 (Gabra4 protein, rat)
RN  - 0 (Receptors, GABA-A)
RN  - 01MI4Q9DI3 (Pilocarpine)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Epilepsy, Temporal Lobe/chemically induced/*metabolism
MH  - Pilocarpine/pharmacology
MH  - Rats, Sprague-Dawley
MH  - Receptors, GABA-A/*metabolism
MH  - Seizures/chemically induced/*metabolism
MH  - gamma-Aminobutyric Acid/*metabolism
PMC - PMC4596257
MID - NIHMS619972
OTO - NOTNLM
OT  - GABAA receptor
OT  - Pilocarpine
OT  - Spontaneous seizure
COIS- Disclosure of Conflicts of Interests: None of the authors has any conflict of 
      interest to disclose. We confirm that we have read the Journal's position on 
      issues involved in ethical publication and affirm that this report is consistent 
      with those guidelines.
EDAT- 2014/09/17 06:00
MHDA- 2015/01/30 06:00
PMCR- 2015/11/01
CRDT- 2014/09/17 06:00
PHST- 2014/07/31 00:00 [accepted]
PHST- 2014/09/17 06:00 [entrez]
PHST- 2014/09/17 06:00 [pubmed]
PHST- 2015/01/30 06:00 [medline]
PHST- 2015/11/01 00:00 [pmc-release]
AID - 10.1111/epi.12771 [doi]
PST - ppublish
SO  - Epilepsia. 2014 Nov;55(11):1826-33. doi: 10.1111/epi.12771. Epub 2014 Sep 15.