PMID- 25224028
OWN - NLM
STAT- MEDLINE
DCOM- 20151116
LR  - 20221207
IS  - 1758-2229 (Electronic)
IS  - 1758-2229 (Linking)
VI  - 7
IP  - 2
DP  - 2015 Apr
TI  - Re-appraisal of the phylogeny and fluorescence in situ hybridization probes for 
      the analysis of the Competibacteraceae in wastewater treatment systems.
PG  - 166-74
LID - 10.1111/1758-2229.12215 [doi]
AB  - Members of the family Competibacteraceae are common in wastewater treatment 
      plants (WWTPs) designed for enhanced biological phosphorus removal (EBPR) and are 
      putatively deleterious to the process of P removal. Their ability to accumulate 
      large amounts of polyhydroxyalkanoates is also suggested to be of potential 
      commercial interest for bioplastic production. In this study we have updated the 
      16S rRNA-based phylogeny of the Competibacter and the Plasticicumulans lineages. 
      The former is delineated by 13 clades including two described genera; 
      'Ca. Competibacter' and 'Ca. Contendobacter'. The oligonucleotide probes used for 
      detection of the family by fluorescence in situ hybridization (FISH) were 
      re-evaluated and designed for coverage of these clades. Surveys of full-scale 
      WWTPs based on 16S rRNA gene amplicon sequencing and FISH analysis indicate that 
      a number of member clades always coexist, with their relative abundances varying 
      substantially between and temporally within plants. The hypothesis that these 
      differences are based on niche partitioning is supported by marked phenotypic 
      differences between clades. An in-depth understanding of the ecology of the 
      family requires further studies of the metabolism of individual clades in situ. 
      The proposed phylogeny and FISH probes will provide the foundation for such 
      studies.
CI  - (c) 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - McIlroy, Simon J
AU  - McIlroy SJ
AD  - Centre for Microbial Communities, Department of Biotechnology, Chemistry and 
      Environmental Engineering, Aalborg University, Aalborg, Denmark.
FAU - Nittami, Tadashi
AU  - Nittami T
FAU - Kanai, Eri
AU  - Kanai E
FAU - Fukuda, Junji
AU  - Fukuda J
FAU - Saunders, Aaron M
AU  - Saunders AM
FAU - Nielsen, Per Halkjaer
AU  - Nielsen PH
LA  - eng
SI  - BioProject/PRJEB5095
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141202
PL  - United States
TA  - Environ Microbiol Rep
JT  - Environmental microbiology reports
JID - 101499207
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Waste Water)
SB  - IM
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Gammaproteobacteria/*classification/*isolation & purification
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Sequence Data
MH  - *Oligonucleotide Probes/genetics
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Sequence Analysis, DNA
MH  - Wastewater/*microbiology
EDAT- 2014/09/17 06:00
MHDA- 2015/11/17 06:00
CRDT- 2014/09/17 06:00
PHST- 2014/06/28 00:00 [received]
PHST- 2014/08/19 00:00 [revised]
PHST- 2014/09/04 00:00 [accepted]
PHST- 2014/09/17 06:00 [entrez]
PHST- 2014/09/17 06:00 [pubmed]
PHST- 2015/11/17 06:00 [medline]
AID - 10.1111/1758-2229.12215 [doi]
PST - ppublish
SO  - Environ Microbiol Rep. 2015 Apr;7(2):166-74. doi: 10.1111/1758-2229.12215. Epub 
      2014 Dec 2.