PMID- 25226066
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150521
LR  - 20141021
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 86
IP  - 20
DP  - 2014 Oct 21
TI  - Development of a simplified microfluidic injector for analysis of droplet content 
      via capillary electrophoresis.
PG  - 10193-200
LID - 10.1021/ac502272q [doi]
AB  - Droplet-based microfluidic platforms sequester nanoliter to picoliter samples in 
      an immiscible carrier phase and have gained notoriety for their ability to be 
      used in laboratory procedures on a miniaturized scale. Recently, droplet 
      microfluidics has been used to prevent zone diffusion in time-resolved sample 
      collection methods and in separation techniques. The assay of droplets remains 
      challenging, however, because the carrier phase is often incompatible with 
      separation techniques. In this work, we report the development of a droplet 
      injector for capillary electrophoresis (CE) which delivers 750 pL droplets to a 
      channel for separation while excluding the fluorous carrier phase. This design is 
      simple compared to previous reports, consisting of only two straight channels and 
      no additional working parts such as membranes or valves. To demonstrate a 
      proof-of-concept and characterize performance, riboflavin was used as a 
      biologically relevant model molecule. Droplets containing a step change in 
      riboflavin concentration were injected and mobilized by CE. The current method is 
      capable of riboflavin peak % relative standard deviations (RSDs) down to 4.4% and 
      temporal resolutions down to 15 s. Human urine samples containing riboflavin and 
      its photolysis products were successfully separated and found to be chemically 
      compatible with the injector. Our simplified design could improve robustness and 
      ruggedness and may allow device construction via nontraditional fabrication 
      techniques.
FAU - DeLaMarre, Michael F
AU  - DeLaMarre MF
AD  - Department of Chemistry, University of Illinois at Chicago , MC 111, 845 West 
      Taylor Street, Chicago, Illinois 60647, United States.
FAU - Shippy, Scott A
AU  - Shippy SA
LA  - eng
PT  - Journal Article
DEP - 20141001
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
EDAT- 2014/09/17 06:00
MHDA- 2014/09/17 06:01
CRDT- 2014/09/17 06:00
PHST- 2014/09/17 06:00 [entrez]
PHST- 2014/09/17 06:00 [pubmed]
PHST- 2014/09/17 06:01 [medline]
AID - 10.1021/ac502272q [doi]
PST - ppublish
SO  - Anal Chem. 2014 Oct 21;86(20):10193-200. doi: 10.1021/ac502272q. Epub 2014 Oct 1.