PMID- 25226906
OWN - NLM
STAT- MEDLINE
DCOM- 20150624
LR  - 20140917
IS  - 1878-3619 (Electronic)
IS  - 0959-2989 (Linking)
VI  - 24
IP  - 6
DP  - 2014
TI  - The Synthesis and characteristic study of transferrin-conjugated liposomes 
      carrying brain-derived neurotrophic factor.
PG  - 2089-99
LID - 10.3233/BME-141019 [doi]
AB  - This study aimed to establish a novel non-viral liposome vector delivering brain 
      derived neurotrophic factor (BDNF) through the blood brain barrier. For this 
      purpose, different water-oil ratios were tested to create liposomes for packaging 
      the prophase synthesized plasmids encoding the BDNF proteins. In order to 
      increase the targeted and peripheral circulation time, we connected the liposomes 
      with transferrin (Tf) and a polyethylene glycol (PEG) long chain. The non-isotope 
      method was used to measure the liposome envelopment ratio and ligand-binding 
      ratio, and also to detect molecular biological features, such as particle size 
      and stability. Tf-conjugated liposomes could be synthesized satisfactorily under 
      the following conditions: the ratio of phospholipid to cholesterol was 1:1; the 
      ratio of enter to plasmid was 100:1; oil phase was dichloromethane; the oil to 
      water ratio was 4:1; the rotary evaporation temperature was 30  degrees C; the ultrasonic 
      temperature was 10  degrees C; the ultrasonic time was 10 min; and 10% trehalose was in 
      the presence. Generated liposomes had a uniform circular shape and particle size 
      distribution. In this experiment, we successfully established a new type of 
      Tf-conjugated liposomes carrying the gene of BDNF and the study provides an 
      experimental basis for the future.
FAU - Chen, Jia-jun
AU  - Chen JJ
AD  - Department of neurology, China-Japan Union Hospital, Jilin University, 126 
      Xiantai Street, Changchun 130012, China.
FAU - Jin, Mei-xiang
AU  - Jin MX
AD  - Department of neurology, Jilin province people's hospital, Changchun 130012, 
      China, 130021.
FAU - Zhu, Shu-lai
AU  - Zhu SL
AD  - Department of neurology, China-Japan Union Hospital, Jilin University, 126 
      Xiantai Street, Changchun 130012, China.
FAU - Li, Fei
AU  - Li F
AD  - Department of neurology, China-Japan Union Hospital, Jilin University, 126 
      Xiantai Street, Changchun 130012, China.
FAU - Xing, Ying
AU  - Xing Y
AD  - Department of neurology, China-Japan Union Hospital, Jilin University, 126 
      Xiantai Street, Changchun 130012, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomed Mater Eng
JT  - Bio-medical materials and engineering
JID - 9104021
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Liposomes)
RN  - 0 (Transferrin)
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*chemistry/genetics/*metabolism
MH  - Drug Compounding/methods
MH  - Drug Design
MH  - Humans
MH  - Liposomes/*chemical synthesis
MH  - Materials Testing
MH  - Plasmids/*administration & dosage/genetics
MH  - Transfection/*methods
MH  - Transferrin/*pharmacokinetics
OTO - NOTNLM
OT  - Brain derived neurotrophic factor
OT  - Tf-conjuaged
OT  - liposome
OT  - non-viral vector
OT  - transferrin
EDAT- 2014/09/18 06:00
MHDA- 2015/06/25 06:00
CRDT- 2014/09/18 06:00
PHST- 2014/09/18 06:00 [entrez]
PHST- 2014/09/18 06:00 [pubmed]
PHST- 2015/06/25 06:00 [medline]
AID - Y4M311129T6M0244 [pii]
AID - 10.3233/BME-141019 [doi]
PST - ppublish
SO  - Biomed Mater Eng. 2014;24(6):2089-99. doi: 10.3233/BME-141019.