PMID- 25228033
OWN - NLM
STAT- MEDLINE
DCOM- 20150615
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 34
IP  - 4
DP  - 2014
TI  - Mechanism of the protective effect of Yulangsan flavonoid on myocardial 
      ischemia/reperfusion injury in rats.
PG  - 1050-62
LID - 10.1159/000366320 [doi]
AB  - AIMS: Effect and mechanism of Yulangsan flavonoid (YLSF) on rat myocardial 
      ischemia/reperfusion injury (MI/RI) has been investigated. METHODS: 
      Sprague-Dawley (SD) rats were randomly divided into seven groups (sham group, 
      model group and NS group: 2 mL of normal saline/kg body weight was administered; 
      diltiazem group: 5 mg of diltiazem hydrochloride/kg body weight was administered; 
      YLSFL, YLSFM and YLSFH groups: 20, 40 and 80 mg of YLSF/kg body weight was 
      administered) and the MI/RI model was established. Myocardial infarct area, 
      levels of myocardial enzymes and nitric oxide synthase (NOS) were measured. 
      Caspase-3 and adenine nucleotide translocator-1 (ANT1) mRNA expression were 
      evaluated by reverse transcription polymerase chain reaction (RT-PCR). 
      Pathological structure and cardiocyte ultrastructure were also analysed. RESULTS: 
      Compared with the MI/RI group, pretreatment with YLSF or diltiazem hydrochloride 
      decreased the infarct area, levels of inducible nitric oxide synthase (iNOS), 
      caspase-3 as well as the leakage of myocardial enzyme and increased activities of 
      total nitric oxide synthase (tNOS) as well as constitutive nitric oxide synthase 
      (cNOS). Cellular edema and the infiltration of inflammatory cells were 
      alleviated. CONCLUSIONS: The experiment showed that YLSF protected the heart 
      against MI/RI, possibly by reducing lipid peroxidation damage, regulating NOS 
      activity and modulating the apoptosis genes expression.
CI  - (c) 2014 S. Karger AG, Basel.
FAU - Zhang, Xudong
AU  - Zhang X
AD  - Department of Pharmacology, Guangxi Medical University, Nanning, PR China.
FAU - Liang, Xingmei
AU  - Liang X
FAU - Lin, Xing
AU  - Lin X
FAU - Zhang, Shijun
AU  - Zhang S
FAU - Huang, Zhongshi
AU  - Huang Z
FAU - Chen, Chunxia
AU  - Chen C
FAU - Guo, Youjia
AU  - Guo Y
FAU - Xuan, Feifei
AU  - Xuan F
FAU - Xu, Xiaohui
AU  - Xu X
FAU - Huang, Renbin
AU  - Huang R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140908
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Flavonoids)
RN  - 0 (Protective Agents)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Caspase 3/metabolism
MH  - Flavonoids/*pharmacology
MH  - Heart/*drug effects
MH  - Lipid Peroxidation/drug effects
MH  - Myocardial Infarction/drug therapy/metabolism
MH  - Myocardial Reperfusion Injury/*drug therapy/metabolism
MH  - Myocardium/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase/metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Protective Agents/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2014/09/18 06:00
MHDA- 2015/06/16 06:00
CRDT- 2014/09/18 06:00
PHST- 2014/07/14 00:00 [accepted]
PHST- 2014/09/18 06:00 [entrez]
PHST- 2014/09/18 06:00 [pubmed]
PHST- 2015/06/16 06:00 [medline]
AID - 000366320 [pii]
AID - 10.1159/000366320 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2014;34(4):1050-62. doi: 10.1159/000366320. Epub 2014 Sep 
      8.