PMID- 25228542
OWN - NLM
STAT- MEDLINE
DCOM- 20141222
LR  - 20211021
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 55
IP  - 10
DP  - 2014 Sep 16
TI  - Nerve fiber layer thinning lags retinal ganglion cell density following crush 
      axonopathy.
PG  - 6505-13
LID - 10.1167/iovs.14-14525 [doi]
AB  - PURPOSE: We investigated the progressive nature of neurodegenerative structural 
      changes following injury to retinal ganglion cell (RGC) axons using quantifiable 
      and noninvasive in vivo imaging techniques. METHODS: To track degenerative RGC 
      progression in retinas following optic nerve crush (ONC) injury, spectral-domain 
      optical coherence tomography (SD-OCT) was used to quantitate the RGC nerve fiber 
      layer (NFL) density. The RGC soma cell density (RCD) was measured by confocal 
      scanning laser ophthalmoscopy (CSLO). The RCD counts were performed using blood 
      vessels as landmarks to anatomically track defined progressive changes in 
      enhanced yellow fluorescent fusion protein (EYFP)-labeled RGCs. RESULTS: 
      Following ONC injury, 68% of the observed decrease in RCD measured by CSLO and 
      54% of the NFL thickness obtained by SD-OCT imaging (N=4 retinas) occurred within 
      the first week. Between days 7 and 14, an additional 22% decrease in RCD was 
      concurrent with a 31% decrease in overall NFL thickness. Finally, between days 14 
      and 21, an additional 10% decrease in RCD measured in vivo by CSLO and 15% 
      decrease in NFL thickness by SD-OCT was observed. CONCLUSIONS: Our data suggest 
      that in vivo CSLO imaging of EYFP-RGC expression and SD-OCT measured NFL 
      thickness are fast and reliable methods that longitudinally track 
      neurodegenerative progression following ONC injury. Neurodegenerative changes in 
      NFL thickness measured by SD-OCT imaging have the same overall trajectory as 
      those observed by CSLO for RCD; however, changes in NFL thickness initially lag 
      behind in vivo RGC soma counts with a slower decline in overall measurable 
      change.
CI  - Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
FAU - Munguba, Gustavo C
AU  - Munguba GC
AD  - Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, 
      Florida, United States.
FAU - Galeb, Sanja
AU  - Galeb S
AD  - Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, 
      Florida, United States.
FAU - Liu, Yuan
AU  - Liu Y
AD  - Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, 
      Florida, United States.
FAU - Landy, David C
AU  - Landy DC
AD  - Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, 
      Florida, United States.
FAU - Lam, Daisy
AU  - Lam D
AD  - Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, 
      Florida, United States.
FAU - Camp, Andrew
AU  - Camp A
AD  - Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, 
      Florida, United States.
FAU - Samad, Sinthia
AU  - Samad S
AD  - Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, 
      Florida, United States.
FAU - Tapia, Mary L
AU  - Tapia ML
AD  - Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, 
      Florida, United States.
FAU - Lee, Richard K
AU  - Lee RK
AD  - Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, 
      Florida, United States.
LA  - eng
GR  - P30 EY014801/EY/NEI NIH HHS/United States
GR  - EY014801/EY/NEI NIH HHS/United States
GR  - F31 EY022872/EY/NEI NIH HHS/United States
GR  - EY016775/EY/NEI NIH HHS/United States
GR  - EY022872/EY/NEI NIH HHS/United States
GR  - K08 EY016775/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140916
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
MH  - Animals
MH  - Axons/*pathology
MH  - Cell Count
MH  - Disease Models, Animal
MH  - Mice
MH  - Mice, Transgenic
MH  - Nerve Crush/adverse effects
MH  - Optic Disk/*pathology
MH  - Optic Nerve Injuries/*pathology
MH  - Retinal Ganglion Cells/*pathology
MH  - Tomography, Optical Coherence
PMC - PMC4201323
OTO - NOTNLM
OT  - glaucoma
OT  - neurodegeneration
OT  - optical coherence tomography
OT  - retinal ganglion cells
OT  - scanning laser ophthalmoscopy
EDAT- 2014/09/18 06:00
MHDA- 2014/12/23 06:00
PMCR- 2015/04/01
CRDT- 2014/09/18 06:00
PHST- 2014/09/18 06:00 [entrez]
PHST- 2014/09/18 06:00 [pubmed]
PHST- 2014/12/23 06:00 [medline]
PHST- 2015/04/01 00:00 [pmc-release]
AID - iovs.14-14525 [pii]
AID - 10.1167/iovs.14-14525 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2014 Sep 16;55(10):6505-13. doi: 
      10.1167/iovs.14-14525.