PMID- 2522906
OWN - NLM
STAT- MEDLINE
DCOM- 19890519
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 66
IP  - 3
DP  - 1989 Mar
TI  - T-cell activation. I. Evidence for a functional linkage between class I MHC 
      antigens and the Tc-Ti complex.
PG  - 348-53
AB  - This paper examines the possibility of a functional linkage between class I MHC 
      molecules and the T-cell receptor complex for antigen (T3-Ti). A newly developed 
      anti-CD3 antibody (500A2) was used as an activation signal for EL4 lymphoma cells 
      and allospecific cytotoxic T-cell clones (CTL), and the production of IL-2/IL-2 
      receptor in EL4 cells and serine esterase in CTL was determined. Anti-CD3 
      antibody-induced activation of both EL4 and CTL cells was enhanced in the 
      presence of immunologically cross-linked and immobilized anti-H-2 (class I) 
      antibody reactive against the H-2 haplotype of the responding T cells. A number 
      of H-2-negative and H-2-positive EL4 subclones were generated and tested for 
      anti-CD3 antibody-induced IL-2/IL-2 receptor production. Although both 
      H-2-positive and -negative subclones expressed CD3 antigen and produced IL-2 
      after activation with the phorbol ester TPA, only the H-2-positive cell clones 
      produced IL-2 and expressed IL-2 receptor after anti-CD3 antibody induction. Our 
      results are compatible with the existence of a functional linkage between the 
      class I and the CD3 molecules on the surface of T cells.
FAU - Brams, P
AU  - Brams P
AD  - Receptron, Concord, California.
FAU - Claesson, M H
AU  - Claesson MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD3 Complex)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Interleukin-2)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Receptors, Interleukin-2)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens, Differentiation, T-Lymphocyte/*immunology
MH  - CD3 Complex
MH  - Clone Cells
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Interleukin-2/biosynthesis
MH  - *Lymphocyte Activation
MH  - Receptors, Antigen, T-Cell/*immunology
MH  - Receptors, Interleukin-2/biosynthesis
MH  - T-Lymphocytes/*immunology/metabolism
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - T-Lymphocytes, Helper-Inducer/immunology
PMC - PMC1385219
EDAT- 1989/03/01 00:00
MHDA- 1989/03/01 00:01
PMCR- 1990/03/01
CRDT- 1989/03/01 00:00
PHST- 1989/03/01 00:00 [pubmed]
PHST- 1989/03/01 00:01 [medline]
PHST- 1989/03/01 00:00 [entrez]
PHST- 1990/03/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1989 Mar;66(3):348-53.