PMID- 25229962
OWN - NLM
STAT- MEDLINE
DCOM- 20150129
LR  - 20140918
IS  - 1008-8830 (Print)
IS  - 1008-8830 (Linking)
VI  - 16
IP  - 9
DP  - 2014 Sep
TI  - [Intracerebral transplantation of human umbilical cord-derived mesenchymal stem 
      cells in neonatal rat model of hypoxic-ischemic brain damage: protective effect 
      to injured brain].
PG  - 927-32
AB  - OBJECTIVE: To study the brain protection and the possible mechanism of human 
      umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in neonatal rat model of 
      hypoxic-ischemic brain damage (HIBD). METHODS: Successfully establishing a 
      neonatal rat model of HIBD, hUC-MSCs labeled with BrdU were transplanted into the 
      lateral ventricle 24 hours after HIBD. The number of apoptotic cells and the 
      expression of Caspase-3 were detected by TUNEL and Western blot respectively at 
      24 and 48 hours after transplantation. The neurological functions of HIBD rats 
      were evaluated by Longa score, and the survival, differentiation and 
      pro-differentiation effects of hUC-MSCs were identified by immunofluorescence at 
      1 to 3 weeks after transplantation. RESULTS: At 24 and 48 hours after 
      transplantation, apoptotic cells and Caspase-3 expression in the MSCs group were 
      less than in the HIBD group (P<0.05). At 2 and 3 weeks after transplantation, the 
      Longa score in the MSCs group was lower than in the HIBD group (P<0.05). After 
      transplantation, positive cells labeled with BrdU were seen in the brain tissue. 
      The expression levels of glial fibrillary acidic protein (GFAP) and neuron 
      specific esterase (NSE) in the MSCs group were higher than in the HIBD and 
      sham-operated control groups (P<0.05), and increased gradually with the 
      transplantation time (P<0.05). CONCLUSIONS: hUC-MSCs transplantation in HIBD rats 
      can inhibit Caspase-3 expression and reduce apoptotic cells in the early stage, 
      and in the later period, the survival hUC-MSCs can differentiate into neural-like 
      cells and promote the differentiation of endogenous neural-like cells, providing 
      protective effects to brain.
FAU - Zhang, De-Shuang
AU  - Zhang DS
AD  - Department of Neonatology, West China Second University Hospital, Chengdu 610041, 
      China. chenjuan2000@163.com.
FAU - Bai, Xiao-Hong
AU  - Bai XH
FAU - Chen, Da-Peng
AU  - Chen DP
FAU - Mu, De-Zhi
AU  - Mu DZ
FAU - Chen, Juan
AU  - Chen J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Dang Dai Er Ke Za Zhi
JT  - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
JID - 100909956
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Apoptosis
MH  - Caspase 3/metabolism
MH  - Cell Differentiation
MH  - *Cord Blood Stem Cell Transplantation
MH  - Disease Models, Animal
MH  - Female
MH  - Hypoxia-Ischemia, Brain/pathology/*therapy
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Phosphopyruvate Hydratase/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2014/09/18 06:00
MHDA- 2015/01/30 06:00
CRDT- 2014/09/18 06:00
PHST- 2014/09/18 06:00 [entrez]
PHST- 2014/09/18 06:00 [pubmed]
PHST- 2015/01/30 06:00 [medline]
AID - 10.7499/j.issn.1008-8830.2014.09.013 [pii]
PST - ppublish
SO  - Zhongguo Dang Dai Er Ke Za Zhi. 2014 Sep;16(9):927-32.