PMID- 25229974
OWN - NLM
STAT- MEDLINE
DCOM- 20141214
LR  - 20141011
IS  - 1938-5404 (Electronic)
IS  - 0033-7587 (Linking)
VI  - 182
IP  - 4
DP  - 2014 Oct
TI  - Induction of chromosomal aberrations at fluences of less than one HZE particle 
      per cell nucleus.
PG  - 368-79
LID - 10.1667/RR13721.1 [doi]
AB  - The assumption of a linear dose response used to describe the biological effects 
      of high-LET radiation is fundamental in radiation protection methodologies. We 
      investigated the dose response for chromosomal aberrations for exposures 
      corresponding to less than one particle traversal per cell nucleus by high-energy 
      charged (HZE) nuclei. Human fibroblast and lymphocyte cells were irradiated with 
      several low doses of <0.1 Gy, and several higher doses of up to 1 Gy with oxygen 
      (77 keV/mum), silicon (99 keV/mum) or Fe (175 keV/mum), Fe (195 keV/mum) or Fe (240 
      keV/mum) particles. Chromosomal aberrations at first mitosis were scored using 
      fluorescence in situ hybridization (FISH) with chromosome specific paints for 
      chromosomes 1, 2 and 4 and DAPI staining of background chromosomes. Nonlinear 
      regression models were used to evaluate possible linear and nonlinear 
      dose-response models based on these data. Dose responses for simple exchanges for 
      human fibroblasts irradiated under confluent culture conditions were best fit by 
      nonlinear models motivated by a nontargeted effect (NTE). The best fits for dose 
      response data for human lymphocytes irradiated in blood tubes were a linear 
      response model for all particles. Our results suggest that simple exchanges in 
      normal human fibroblasts have an important NTE contribution at low-particle 
      fluence. The current and prior experimental studies provide important evidence 
      against the linear dose response assumption used in radiation protection for HZE 
      particles and other high-LET radiation at the relevant range of low doses.
FAU - Hada, Megumi
AU  - Hada M
AD  - a  Universities Space Research Association, Houston, Texas 77058.
FAU - Chappell, Lori J
AU  - Chappell LJ
FAU - Wang, Minli
AU  - Wang M
FAU - George, Kerry A
AU  - George KA
FAU - Cucinotta, Francis A
AU  - Cucinotta FA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140917
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Cell Nucleus/*genetics/*radiation effects
MH  - Chromosome Aberrations/*radiation effects
MH  - Cosmic Radiation/*adverse effects
MH  - Dose-Response Relationship, Radiation
MH  - Endpoint Determination
MH  - Fibroblasts/cytology/radiation effects
MH  - Humans
MH  - Linear Energy Transfer
EDAT- 2014/09/18 06:00
MHDA- 2014/12/17 06:00
CRDT- 2014/09/18 06:00
PHST- 2014/09/18 06:00 [entrez]
PHST- 2014/09/18 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
AID - 10.1667/RR13721.1 [doi]
PST - ppublish
SO  - Radiat Res. 2014 Oct;182(4):368-79. doi: 10.1667/RR13721.1. Epub 2014 Sep 17.