PMID- 25230382
OWN - NLM
STAT- MEDLINE
DCOM- 20160318
LR  - 20211021
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Print)
IS  - 1552-4973 (Linking)
VI  - 103
IP  - 5
DP  - 2015 Jul
TI  - Incorporation of copper into chitosan scaffolds promotes bone regeneration in rat 
      calvarial defects.
PG  - 1044-9
LID - 10.1002/jbm.b.33290 [doi]
AB  - The objective of this study was to investigate the effects of a copper loaded 
      chitosan scaffold on bone regeneration in critical-sized calvarial defects in 
      rats. Chitosan scaffolds and copper-chitosan scaffolds were fabricated and 
      characterized by scanning electron microscopy (SEM). Chitosan and copper-chitosan 
      scaffolds were implanted into 5 mm diameter critical-sized calvarial defects in 
      Fisher 344 male rats. Empty defects (no scaffolds) were included as a control. 
      After 4 weeks, the rats were sacrificed for microcomputed tomography (micro-CT) 
      and histological analysis of new bone tissue development. Microscopy images 
      revealed the uniformly porous structure of chitosan and copper-chitosan 
      scaffolds. Significant bone regeneration was noted in the defects treated with 
      copper-chitosan scaffolds when evaluated using micro-CT and histological 
      analysis, when compared with other groups tested. On analysis of the micro-CT 
      scans, an eleven-fold and a two-fold increase in the new bone volume/total volume 
      (BV/TV) % was found in defects treated with the copper-chitosan scaffolds, when 
      compared to empty defects and chitosan scaffolds, respectively. This study 
      demonstrated the suitability of copper-crosslinked chitosan scaffolds for bone 
      tissue engineering and provides the first evidence that inclusion of copper ions 
      in scaffolds can enhance tissue regeneration.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - D'Mello, Sheetal
AU  - D'Mello S
AD  - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, 
      University of Iowa, Iowa, 52242.
FAU - Elangovan, Satheesh
AU  - Elangovan S
AD  - Department of Periodontics, College of Dentistry, University of Iowa, Iowa City, 
      Iowa, 52242.
FAU - Hong, Liu
AU  - Hong L
AD  - Department of Prosthodontics, College of Dentistry, University of Iowa, Iowa 
      City, Iowa.
FAU - Ross, Ryan D
AU  - Ross RD
AD  - Department of Anatomy and Cell Biology, Rush Medical College, Chicago, Illinois.
FAU - Sumner, D Rick
AU  - Sumner DR
AD  - Department of Anatomy and Cell Biology, Rush Medical College, Chicago, Illinois.
FAU - Salem, Aliasger K
AU  - Salem AK
AD  - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, 
      University of Iowa, Iowa, 52242.
AD  - Department of Periodontics, College of Dentistry, University of Iowa, Iowa City, 
      Iowa, 52242.
LA  - eng
GR  - R21 CA128414/CA/NCI NIH HHS/United States
GR  - R21 CA133456/CA/NCI NIH HHS/United States
GR  - 1R21CA13345-01/R21CA128414-01A2/UI/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140917
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 789U1901C5 (Copper)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - *Bone Regeneration
MH  - Chitosan/*chemistry
MH  - Copper/*chemistry
MH  - *Fracture Healing
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - Skull/*injuries
MH  - Tissue Scaffolds/*chemistry
PMC - PMC4363304
MID - NIHMS667311
OTO - NOTNLM
OT  - biodegradable scaffolds
OT  - bone regeneration
OT  - chitosan
OT  - copper
OT  - tissue engineering
COIS- We have no conflicts of interest to declare.
EDAT- 2014/09/18 06:00
MHDA- 2016/03/19 06:00
PMCR- 2015/10/01
CRDT- 2014/09/18 06:00
PHST- 2014/04/02 00:00 [received]
PHST- 2014/08/03 00:00 [revised]
PHST- 2014/09/01 00:00 [accepted]
PHST- 2014/09/18 06:00 [entrez]
PHST- 2014/09/18 06:00 [pubmed]
PHST- 2016/03/19 06:00 [medline]
PHST- 2015/10/01 00:00 [pmc-release]
AID - 10.1002/jbm.b.33290 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2015 Jul;103(5):1044-9. doi: 
      10.1002/jbm.b.33290. Epub 2014 Sep 17.