PMID- 25231002
OWN - NLM
STAT- MEDLINE
DCOM- 20150223
LR  - 20211021
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 47
IP  - 6
DP  - 2014 Dec
TI  - CD29 of human umbilical cord mesenchymal stem cells is required for expansion of 
      CD34(+) cells.
PG  - 596-603
LID - 10.1111/cpr.12130 [doi]
AB  - OBJECTIVES: Human umbilical cord mesenchymal stem cells (hUCMSCs) play a critical 
      role in expanding haematopoietic stem cells (HSCs) by providing the essential 
      microenvironment for haematopoiesis. In this study, we sought to investigate 
      whether CD29 of hUCMSCs would play a key role in the ability of hUCMSCs to help 
      expand HSCs in vivo and in vitro. MATERIAL AND METHODS: To investigate whether 
      CD29 of hUCMSCs would play a key role for the ability of hUCMSCs to expand HSCs, 
      soluble anti-CD29 antibody was added to co-cultures of hUCMSCs and cord blood 
      (CB) CD34(+) cells. It significantly blocked expansion of CB CD34(+) cells 
      induced by hUCMSCs. Using CD29-deficient hUCMSCs models, long-term 
      culture-initiating cell and non-obese diabetic/severe combined immunodeficient 
      disease mouse repopulating cell assay, revealed that CB CD34(+) cells co-cultured 
      with CD29-deficient hUCMSCs only retained the capacity of multipotent 
      differentiation for 5 weeks at the most. RESULTS: Soluble anti-CD29 antibody 
      significantly blocked expansion of CB CD34(+) cells induced by hUCMSCs. CB 
      CD34(+) cells co-cultured with CD29-deficient hUCMSCs only retained the capacity 
      of multipotent differentiation for 5 weeks at the most. CONCLUSIONS: CB CD34(+) 
      cells co-cultured with CD29-deficient hUCMSCs gave rise to all major 
      haematopoietic lineages, but failed to engraft long term.
CI  - (c) 2014 John Wiley & Sons Ltd.
FAU - Yang, Y
AU  - Yang Y
AD  - Department of Medical Genetics, School of Basic Medical Sciences, Peking 
      University, Beijing, 100191, China.
FAU - Hu, M
AU  - Hu M
FAU - Zhang, Y
AU  - Zhang Y
FAU - Li, H
AU  - Li H
FAU - Miao, Z
AU  - Miao Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140918
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (Antigens, CD34)
RN  - 0 (Integrin beta1)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/*metabolism
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Fetal Blood/*cytology
MH  - Hematopoiesis
MH  - Humans
MH  - Integrin beta1/chemistry/genetics/*metabolism
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mice
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - Transplantation, Heterologous
PMC - PMC6495839
EDAT- 2014/09/19 06:00
MHDA- 2015/02/24 06:00
PMCR- 2014/09/18
CRDT- 2014/09/19 06:00
PHST- 2014/05/28 00:00 [received]
PHST- 2014/06/27 00:00 [accepted]
PHST- 2014/09/19 06:00 [entrez]
PHST- 2014/09/19 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
PHST- 2014/09/18 00:00 [pmc-release]
AID - CPR12130 [pii]
AID - 10.1111/cpr.12130 [doi]
PST - ppublish
SO  - Cell Prolif. 2014 Dec;47(6):596-603. doi: 10.1111/cpr.12130. Epub 2014 Sep 18.