PMID- 25231381
OWN - NLM
STAT- MEDLINE
DCOM- 20150219
LR  - 20141014
IS  - 1520-4995 (Electronic)
IS  - 0006-2960 (Linking)
VI  - 53
IP  - 40
DP  - 2014 Oct 14
TI  - Effects of proton motive force on the structure and dynamics of bovine cytochrome 
      C oxidase in phospholipid vesicles.
PG  - 6382-91
LID - 10.1021/bi501022j [doi]
AB  - A conventional method for reconstituting cytochrome c oxidase (CcO) into 
      phospholipid vesicles (COV) has been modified to permit resonance Raman (RR) 
      analysis in the presence and absence of proton motive force (DeltamuH(+)). The COV has 
      an average diameter of 20 nm and contains one CcO molecule within a unified 
      orientation with CuA located outside the COV. The process of generation of DeltamuH(+) 
      across the membrane was monitored spectrophotometrically with rhodamine123 dye. 
      The COV exhibits a respiratory control ratio (RCR) value of >30 and is tolerant 
      to RR measurements with 10 mW laser illumination for 60 min at 441.6 nm. 
      Structural perturbations at the heme sites caused by incorporation into vesicles 
      were clarified by spectral comparisons between solubilized CcO and COV. 
      Absorption spectroscopy revealed that the rate of electron transfer from 
      cytochrome c to O2 is reduced significantly more in the presence of DeltamuH(+) than 
      in its absence. RR spectroscopic measurements indicate that CcO in COV in the 
      "respiratory-controlled" state adopts a mixed-valence state (heme a(2+) and heme 
      a3(3+)). This study establishes a supramolecular model system for experimentally 
      examining the energy conversion protein machinery in the presence of DeltamuH(+).
FAU - Nomura, Takashi
AU  - Nomura T
AD  - Laboratory of Spectroscopy, Graduate School of Life Science, University of Hyogo 
      , RSC-UH Leading Program Center, Koto 1-1-1, Sayo-cho, Sayo-gun, Hyogo 679-5148, 
      Japan.
FAU - Yanagisawa, Sachiko
AU  - Yanagisawa S
FAU - Shinzawa-Itoh, Kyoko
AU  - Shinzawa-Itoh K
FAU - Yoshikawa, Shinya
AU  - Yoshikawa S
FAU - Ogura, Takashi
AU  - Ogura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141002
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Lipid Bilayers)
RN  - 0 (Phospholipids)
RN  - 0 (Proton Ionophores)
RN  - 18535-39-2 (heme a)
RN  - 42VZT0U6YR (Heme)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Electron Transport
MH  - Electron Transport Complex IV/chemistry/*physiology
MH  - Heme/analogs & derivatives
MH  - Kinetics
MH  - Lipid Bilayers/chemistry
MH  - Membrane Potential, Mitochondrial
MH  - Mitochondria, Heart/drug effects/physiology
MH  - Oxidation-Reduction
MH  - Phospholipids/*chemistry
MH  - Protein Structure, Quaternary
MH  - Proton Ionophores/pharmacology
MH  - *Proton-Motive Force
MH  - Spectrum Analysis, Raman
EDAT- 2014/09/19 06:00
MHDA- 2015/02/20 06:00
CRDT- 2014/09/19 06:00
PHST- 2014/09/19 06:00 [entrez]
PHST- 2014/09/19 06:00 [pubmed]
PHST- 2015/02/20 06:00 [medline]
AID - 10.1021/bi501022j [doi]
PST - ppublish
SO  - Biochemistry. 2014 Oct 14;53(40):6382-91. doi: 10.1021/bi501022j. Epub 2014 Oct 
      2.