PMID- 25231528
OWN - NLM
STAT- MEDLINE
DCOM- 20150617
LR  - 20190221
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 34
IP  - 5
DP  - 2014 Nov
TI  - Hypofractionated radiotherapy induces miR-34a expression and enhances apoptosis 
      in human nasopharyngeal carcinoma cells.
PG  - 1388-94
LID - 10.3892/ijmm.2014.1937 [doi]
AB  - Nasopharyngeal carcinoma (NPC) is a relatively radiosensitive disease. However, 
      the therapeutic effects of radiotherapy are not always satisfactory due to 
      radioresistance. The hypofractionated schema is currently widely used in clinical 
      practice. In the present study, we investigated the effects of hypofractionated 
      radiotherapy on NPC cells and explored the mechanisms involved. In addition, we 
      aimed to determine the role of miR-34a in the effects of hypofractionated 
      radiotherapy and whether these effects occur in a p53-dependent manner. For this 
      purpose, we used CNE1 and CNE2 NPC cells which were subjected to 
      hyperfractionated and hypofractionated radiotherapy. The viability of the cells 
      was measured by MTT assay and acridine orange (AO) and ethidium bromide (EB) 
      staining was used to observe morphological changes. In addition, 
      Annexin V-propidium iodide (PI) staining and flow cytometry were used to 
      determine the number of apoptotic cells and mRNA and protein expression was 
      measured by qPCR and western blot analysis, respectively. The results revealed 
      that hypofractionated radiotherapy enhanced apoptosis and increased the 
      expression of miR-34a and p53 in the NPC cells. In addition, it stimulated p53 
      promoter activity and downregulated the protein expression of c-Myc in the human 
      NPC cells. Furthermore, the knockdown of miR-34a suppressed the growth inhibitory 
      effects induced by hypofractionated radiotherapy. Thus, our results suggest that 
      the enhanced apoptosis of NPC cells may be associated with the miR-34a-mediated 
      suppression of c-Myc in a p53-dependent manner.
FAU - Long, Zhixiong
AU  - Long Z
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei 430060, P.R. China.
FAU - Wang, Bin
AU  - Wang B
AD  - Department of Otolaryngology (ENT), The Fifth Hospital of Wuhan, Wuhan, Hubei 
      430051, P.R. China.
FAU - Tao, Dan
AU  - Tao D
AD  - Department of Oncology, The Fifth Hospital of Wuhan, Wuhan, Hubei 430051, P.R. 
      China.
FAU - Huang, Ying
AU  - Huang Y
AD  - Department of Oncology, The Fifth Hospital of Wuhan, Wuhan, Hubei 430051, P.R. 
      China.
FAU - Tao, Zezhang
AU  - Tao Z
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei 430060, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20140917
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (MIRN34 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Apoptosis/*radiation effects
MH  - Carcinoma
MH  - Cell Line, Tumor
MH  - Cell Survival/radiation effects
MH  - Down-Regulation
MH  - Gene Expression Regulation, Neoplastic/*radiation effects
MH  - Humans
MH  - MicroRNAs/genetics/*metabolism
MH  - Nasopharyngeal Carcinoma
MH  - Nasopharyngeal Neoplasms/*genetics/*radiotherapy
MH  - Promoter Regions, Genetic
MH  - Proto-Oncogene Proteins c-myc/genetics/metabolism
MH  - Radiation Tolerance
MH  - Tumor Suppressor Protein p53/genetics/metabolism
EDAT- 2014/09/19 06:00
MHDA- 2015/06/18 06:00
CRDT- 2014/09/19 06:00
PHST- 2014/04/26 00:00 [received]
PHST- 2014/09/10 00:00 [accepted]
PHST- 2014/09/19 06:00 [entrez]
PHST- 2014/09/19 06:00 [pubmed]
PHST- 2015/06/18 06:00 [medline]
AID - 10.3892/ijmm.2014.1937 [doi]
PST - ppublish
SO  - Int J Mol Med. 2014 Nov;34(5):1388-94. doi: 10.3892/ijmm.2014.1937. Epub 2014 Sep 
      17.