PMID- 25232383
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140918
LR  - 20211021
IS  - 1940-5901 (Print)
IS  - 1940-5901 (Electronic)
IS  - 1940-5901 (Linking)
VI  - 7
IP  - 8
DP  - 2014
TI  - Okadaic acid inhibits cell multiplication and induces apoptosis in a549 cells, a 
      human lung adenocarcinoma cell line.
PG  - 2025-30
AB  - This essay aims to research the effect of okadaic acid (OA) on A549 cell 
      multiplication, and cell apoptosis induced by OA was observed by cell morphology. 
      MTT assay, trypan blue exclusion test (TBET), Giemsa staining method and acridine 
      orange (AO) fluorescence staining assay were applied. The results of cell 
      survival evaluated by TBET and colorimetric assay with 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) showed: The 
      number of A549 cells was decreased in a dose-dependent manner. Cytomorphology 
      observation of okadaic acid-treated cells showed that cells became shrinkage and 
      turned round, some cells floated in the nutrient medium with nucleus 
      agglutination broken, resulting in apoptotic bodies. Above-mentioned results 
      indicated that OA exerted significantly inhibitory effect on A549 cell 
      multiplication due to the apoptosis induced by OA.
FAU - Wang, Renjun
AU  - Wang R
AD  - School of Life Sciences, Qufu Normal University Qufu, Shandong 273165, China.
FAU - Lv, Lili
AU  - Lv L
AD  - School of Life Sciences, Qufu Normal University Qufu, Shandong 273165, China.
FAU - Zhao, Yunfeng
AU  - Zhao Y
AD  - School of Life Sciences, Qufu Normal University Qufu, Shandong 273165, China.
FAU - Yang, Nana
AU  - Yang N
AD  - School of Life Sciences, Qufu Normal University Qufu, Shandong 273165, China.
LA  - eng
PT  - Journal Article
DEP - 20140815
PL  - United States
TA  - Int J Clin Exp Med
JT  - International journal of clinical and experimental medicine
JID - 101471010
PMC - PMC4161543
OTO - NOTNLM
OT  - A549 cell line
OT  - Okadaic acid
OT  - apoptosis
OT  - cell multiplication
EDAT- 2014/09/19 06:00
MHDA- 2014/09/19 06:01
PMCR- 2014/08/15
CRDT- 2014/09/19 06:00
PHST- 2014/05/26 00:00 [received]
PHST- 2014/07/09 00:00 [accepted]
PHST- 2014/09/19 06:00 [entrez]
PHST- 2014/09/19 06:00 [pubmed]
PHST- 2014/09/19 06:01 [medline]
PHST- 2014/08/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Med. 2014 Aug 15;7(8):2025-30. eCollection 2014.