PMID- 25238939
OWN - NLM
STAT- MEDLINE
DCOM- 20150908
LR  - 20220409
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 210
IP  - 12
DP  - 2014 Dec
TI  - Recurrence of lung adenocarcinoma after an interval of 15 years revealed by 
      demonstration of the same type of EML4-ALK fusion gene.
PG  - 1112-6
LID - S0344-0338(14)00229-5 [pii]
LID - 10.1016/j.prp.2014.08.002 [doi]
AB  - We carried out an experiment on a 58-year-old man with multiple left lung tumors 
      and swelling of multiple lymph nodes. For clinical staging and therapeutic 
      purposes, bronchoalveolar lavage (BAL) cytology and lung biopsy were performed. 
      The biopsy specimen revealed the left lower lung mass to be immunohistochemically 
      ALK (anaplastic lymphoma kinase)-positive adenocarcinoma. Using the BAL specimen 
      from the left lower lung, EML4 (echinoderm microtubule-associated protein-like 
      4)-ALK variant 1 fusion gene was detected by reverse transcription-polymerase 
      chain reaction (RT-PCR). His past history showed that he had undergone an 
      operation for lung adenocarcinoma of the right lower lobe 15 years before, and 
      the pathological specimen at that time revealed that the lung adenocarcinoma with 
      pleural invasion and single metastasis of mediastinal lymph node showed a 
      mucinous cribriform pattern and/or signet-ring cell pattern. The typical 
      histology led us to examine the ALK rearrangement in the primary lung cancer and 
      mediastinal metastatic tumor. Immunohistochemistry (IHC) for ALK was positive, 
      and ALK break apart fluorescence in situ hybridization (FISH) showed a positive 
      result. Moreover, RT-PCR using formalin-fixed, paraffin-embedded tissue from the 
      right lung cancer also demonstrated EML4-ALK variant 1 fusion gene. Although 
      there is a possibility that the left lung cancer is de novo one with multiple 
      metastases, detection of the same fusion gene of the very rare EML4-ALK variant 1 
      in both tumors suggests that the left cancer is a recurrence of the right lung 
      cancer after an interval of 15 years.
CI  - Copyright (c) 2014 Elsevier GmbH. All rights reserved.
FAU - Tsukamoto, Yoshitane
AU  - Tsukamoto Y
AD  - Department of Surgical Pathology Hyogo College of Medicine, 1-1 Mukogawa-cho, 
      Nishinomiya, Hyogo 663-8501, Japan. Electronic address: tsuka-y@hyo-med.ac.jp.
FAU - Kanamori, Kiyonobu
AU  - Kanamori K
AD  - Division of Respiratory Medicine, Department of Internal Medicine, Kitaharima 
      General Medical Center, 926-250 Ichiba-cho, Ono, Hyogo 675-1392, Japan.
FAU - Watanabe, Takahiro
AU  - Watanabe T
AD  - Department of Surgical Pathology Hyogo College of Medicine, 1-1 Mukogawa-cho, 
      Nishinomiya, Hyogo 663-8501, Japan.
FAU - Mikami, Koji
AU  - Mikami K
AD  - Division of Respiratory Medicine, Department of Internal Medicine, Hyogo College 
      of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan.
FAU - Ieki, Ryuji
AU  - Ieki R
AD  - Division of Respiratory Medicine, Department of Internal Medicine, Hyogo College 
      of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan.
FAU - Nakano, Takashi
AU  - Nakano T
AD  - Division of Respiratory Medicine, Department of Internal Medicine, Hyogo College 
      of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan.
FAU - Kajimoto, Kazuyoshi
AU  - Kajimoto K
AD  - Department of Pathology, Hyogo Cancer Center, 13-70 Kitaohji-cho, Akashi, Hyogo 
      673-8558, Japan.
FAU - Hirota, Seiichi
AU  - Hirota S
AD  - Department of Surgical Pathology Hyogo College of Medicine, 1-1 Mukogawa-cho, 
      Nishinomiya, Hyogo 663-8501, Japan.
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20140819
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (EML4-ALK fusion protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
SB  - IM
MH  - Adenocarcinoma/*diagnosis/*genetics
MH  - Adenocarcinoma of Lung
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Lung Neoplasms/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Recurrence
OTO - NOTNLM
OT  - EML-ALK fusion gene
OT  - Lung cancer
OT  - RT-PCR
OT  - Recurrence
EDAT- 2014/09/23 06:00
MHDA- 2015/09/09 06:00
CRDT- 2014/09/21 06:00
PHST- 2014/07/11 00:00 [received]
PHST- 2014/07/31 00:00 [revised]
PHST- 2014/08/06 00:00 [accepted]
PHST- 2014/09/21 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2015/09/09 06:00 [medline]
AID - S0344-0338(14)00229-5 [pii]
AID - 10.1016/j.prp.2014.08.002 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2014 Dec;210(12):1112-6. doi: 10.1016/j.prp.2014.08.002. Epub 
      2014 Aug 19.