PMID- 25239414
OWN - NLM
STAT- MEDLINE
DCOM- 20141201
LR  - 20220317
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 142
IP  - 4
DP  - 2014 Oct
TI  - Comparison study of the eosin-5'-maleimide binding test, flow cytometric osmotic 
      fragility test, and cryohemolysis test in the diagnosis of hereditary 
      spherocytosis.
PG  - 474-84
LID - 10.1309/AJCPO7V4OGXLIIPP [doi]
AB  - OBJECTIVES: Current guidelines recommend the eosin-5'-maleimide (EMA) binding 
      test and cryohemolysis test for screening for hereditary spherocytosis (HS), and 
      the flow cytometric osmotic fragility (FC OF) test was recently developed to 
      replace the classic OF test. We evaluatedthe performance of the EMA binding test, 
      FC OF test, cryohemolysis test, and the hemoglobin (Hb)/mean corpuscular 
      hemoglobin concentration (MCHC) ratio in the diagnosis of HS and assessed whether 
      these tests reflect the clinical severity of HS. METHODS: A total of 153 patients 
      with anemia (33 with HS, 40 with autoimmune hemolytic anemia, 40 with anemia of 
      chronic disease, and 40 with iron deficiency anemia [IDA]) and 140 healthy 
      controls were enrolled, and the performance of the three tests was evaluated. 
      RESULTS: Both the EMA binding test (area under the curve [AUC], 0.996) and the FC 
      OF test (AUC, 0.992) performed satisfactorily, but the cryohemolysis test (AUC, 
      0.723) performed significantly worse because of false positivity in patients with 
      IDA. The Hb/MCHC ratio (P < .001) was able to reflect the clinical severity of 
      HS. CONCLUSIONS: Our results demonstrate that both the EMA binding and FC OF 
      tests are useful as screening tests for the diagnosis of HS, but the 
      cryohemolysis test has limited use due to its false positivity in IDA, with the 
      Hb/MCHC ratio the most useful parameter for assessing the clinical severity of 
      HS.
CI  - Copyright(c) by the American Society for Clinical Pathology.
FAU - Park, Sang Hyuk
AU  - Park SH
AD  - From the Department of Laboratory Medicine, University of Ulsan College of 
      Medicine and Asan Medical Center, Seoul, Korea;
FAU - Park, Chan-Jeoung
AU  - Park CJ
AD  - From the Department of Laboratory Medicine, University of Ulsan College of 
      Medicine and Asan Medical Center, Seoul, Korea; cjpark@amc.seoul.kr.
FAU - Lee, Bo-Ra
AU  - Lee BR
AD  - From the Department of Laboratory Medicine, University of Ulsan College of 
      Medicine and Asan Medical Center, Seoul, Korea;
FAU - Cho, Young-Uk
AU  - Cho YU
AD  - From the Department of Laboratory Medicine, University of Ulsan College of 
      Medicine and Asan Medical Center, Seoul, Korea;
FAU - Jang, Seongsoo
AU  - Jang S
AD  - From the Department of Laboratory Medicine, University of Ulsan College of 
      Medicine and Asan Medical Center, Seoul, Korea;
FAU - Kim, Nayoung
AU  - Kim N
AD  - Asan Institute for Life Science, Asan Medical Center, Seoul, Korea;
FAU - Koh, Kyung-Nam
AU  - Koh KN
AD  - Department of Pediatrics, University of Ulsan College of Medicine and Asan 
      Medical Center, Seoul, Korea;
FAU - Im, Ho-Joon
AU  - Im HJ
AD  - Department of Pediatrics, University of Ulsan College of Medicine and Asan 
      Medical Center, Seoul, Korea;
FAU - Seo, Jong-Jin
AU  - Seo JJ
AD  - Department of Pediatrics, University of Ulsan College of Medicine and Asan 
      Medical Center, Seoul, Korea;
FAU - Park, Eun Sil
AU  - Park ES
AD  - Department of Pediatrics, Gyeongsang National University, School of Medicine, 
      Gyeongsang Institute of Health Science, Jinju, Korea;
FAU - Lee, Ji Won
AU  - Lee JW
AD  - Department of Pediatrics, Seoul National University College of Medicine, Seoul, 
      Korea; and.
FAU - Yoo, Keon Hee
AU  - Yoo KH
AD  - Department of Pediatrics, Sungkyunkwan University School of Medicine, Seoul, 
      Korea.
FAU - Jung, Hye Lim
AU  - Jung HL
AD  - Department of Pediatrics, Sungkyunkwan University School of Medicine, Seoul, 
      Korea.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 76296-42-9 (eosin maleimide)
RN  - TDQ283MPCW (Eosine Yellowish-(YS))
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Child, Preschool
MH  - Clinical Laboratory Techniques/*methods
MH  - Diagnosis, Differential
MH  - Eosine Yellowish-(YS)/*analogs & derivatives/metabolism
MH  - False Positive Reactions
MH  - Female
MH  - Flow Cytometry
MH  - Hematologic Tests
MH  - Hemolysis
MH  - Humans
MH  - Infant
MH  - Male
MH  - Middle Aged
MH  - Osmotic Fragility
MH  - Reference Values
MH  - Sensitivity and Specificity
MH  - Spherocytosis, Hereditary/*diagnosis/metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - Cryohemolysis test
OT  - EMA binding test
OT  - Flow cytometric osmotic fragility test
OT  - Hb/MCHC
OT  - Hereditary spherocytosis
EDAT- 2014/09/23 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/09/21 06:00
PHST- 2014/09/21 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 142/4/474 [pii]
AID - 10.1309/AJCPO7V4OGXLIIPP [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2014 Oct;142(4):474-84. doi: 10.1309/AJCPO7V4OGXLIIPP.