PMID- 25239752
OWN - NLM
STAT- MEDLINE
DCOM- 20150521
LR  - 20140920
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1227
DP  - 2015
TI  - New BAC probe set to narrow down chromosomal breakpoints in small and large 
      derivative chromosomes, especially suited for mosaic conditions.
PG  - 279-87
LID - 10.1007/978-1-4939-1652-8_14 [doi]
AB  - Fluorescence in situ hybridization (FISH) and/or array-comparative genomic 
      hybridization (aCGH) performed after initial banding cytogenetics is still the 
      gold standard for detection of chromosomal rearrangements. Although aCGH provides 
      a higher resolution, FISH has two main advantages over the array-based 
      approaches: (1) it can be applied to characterize balanced as well as unbalanced 
      rearrangements, whereas aCGH is restricted to unbalanced ones, and (2) 
      chromosomal aberrations present in low level or complex mosaics can be 
      characterized by FISH without any problems, while aCGH requires presence of over 
      50 % of aberrant cells in the sample for detection. Recently, a new FISH-based 
      probe set was presented: the so-called pericentric-ladder-FISH (PCL-FISH) that 
      enables characterization of chromosomal breakpoints especially in mosaic small 
      supernumerary marker chromosomes (sSMC). It can also be applied on large inborn 
      or acquired derivative chromosomes. The main feature of this set is that the 
      probes are applied in a chromosome-specific manner and they align along the 
      chromosome in average intervals of ten megabasepairs. Hence PCL-FISH provides 
      denser coverage and a more precise anchorage on the human DNA-sequence than most 
      other FISH-banding approaches.
FAU - Hamid, Ahmed B
AU  - Hamid AB
AD  - Jena University Hospital, Institute of Human Genetics, Friedrich Schiller 
      University, Kollegiengasse 10, Jena, D-07743, Germany.
FAU - Fan, Xiaobo
AU  - Fan X
FAU - Kosyakova, Nadezda
AU  - Kosyakova N
FAU - Radhakrishnan, Gopakumar
AU  - Radhakrishnan G
FAU - Liehr, Thomas
AU  - Liehr T
FAU - Karamysheva, Tatyana
AU  - Karamysheva T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Molecular Probes)
SB  - IM
MH  - Chromosome Breakpoints
MH  - Chromosomes, Artificial, Bacterial/*chemistry/genetics
MH  - Comparative Genomic Hybridization
MH  - Cytogenetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Probes/*chemistry/genetics
MH  - *Mosaicism
EDAT- 2014/09/23 06:00
MHDA- 2015/05/23 06:00
CRDT- 2014/09/21 06:00
PHST- 2014/09/21 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
AID - 10.1007/978-1-4939-1652-8_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1227:279-87. doi: 10.1007/978-1-4939-1652-8_14.