PMID- 25239753
OWN - NLM
STAT- MEDLINE
DCOM- 20150521
LR  - 20140920
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1227
DP  - 2015
TI  - BAC-probes applied for characterization of fragile sites (FS).
PG  - 289-98
LID - 10.1007/978-1-4939-1652-8_15 [doi]
AB  - Genomic instability tends to occur at specific genomic regions known as common 
      fragile sites (FS). FS are evolutionarily conserved and generally involve late 
      replicating regions with AT-rich sequences. The possible correlation between some 
      FS and cancer-related breakpoints emphasizes on the importance of understanding 
      the mechanisms of chromosomal instability at these sites. Although about 230 FS 
      have already been mapped cytogenetically, only a few of them have been 
      characterized on a molecular level. In this chapter, we provide a protocol for 
      mapping of common FS using bacterial artificial chromosome (BAC) probes in 
      fluorescence in situ hybridization (FISH) and suggest the usage of lymphocytes 
      from Fanconi anemia patients as a model system. In the latter, rare FS are 
      expressed much more frequently than in, for example, aphidicolin-induced blood 
      lymphocyte preparations. Knowing the exact location of FS enables the molecular 
      comparison of their location and breakpoints that appear during evolution, cancer 
      development and inherited disorders.
FAU - Mrasek, Kristin
AU  - Mrasek K
AD  - Jena University Hospital, Institute of Human Genetics, Friedrich Schiller 
      University, Kollegiengasse 10, Jena, D-07743, Germany.
FAU - Wilhelm, Kathleen
AU  - Wilhelm K
FAU - Quintana, Luciana G
AU  - Quintana LG
FAU - Theuss, Luise
AU  - Theuss L
FAU - Liehr, Thomas
AU  - Liehr T
FAU - Leskovac, Andreja
AU  - Leskovac A
FAU - Filipovic, Jelena
AU  - Filipovic J
FAU - Joksic, Gordana
AU  - Joksic G
FAU - Joksic, Ivana
AU  - Joksic I
FAU - Weise, Anja
AU  - Weise A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Molecular Probes)
RN  - 0 (Mutagens)
RN  - 38966-21-1 (Aphidicolin)
SB  - IM
MH  - Aphidicolin/toxicity
MH  - Cell Line
MH  - *Chromosome Fragile Sites
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial/*chemistry/genetics
MH  - Fanconi Anemia/*genetics/metabolism/pathology
MH  - *Genome, Human
MH  - Genomic Instability
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/chemistry/drug effects/pathology
MH  - Molecular Probes/*chemistry/genetics
MH  - Mutagens/toxicity
EDAT- 2014/09/23 06:00
MHDA- 2015/05/23 06:00
CRDT- 2014/09/21 06:00
PHST- 2014/09/21 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2015/05/23 06:00 [medline]
AID - 10.1007/978-1-4939-1652-8_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1227:289-98. doi: 10.1007/978-1-4939-1652-8_15.