PMID- 25239813
OWN - NLM
STAT- MEDLINE
DCOM- 20150825
LR  - 20150129
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 23
IP  - 1
DP  - 2014 Nov
TI  - The anti-inflammation effect of baicalin on experimental colitis through 
      inhibiting TLR4/NF-kappaB pathway activation.
PG  - 294-303
LID - S1567-5769(14)00346-4 [pii]
LID - 10.1016/j.intimp.2014.09.005 [doi]
AB  - BACKGROUND: Baicalin holds a protective effect on inflammatory responses in 
      several diseases. However, its molecular mechanism of anti-inflammatory activity 
      on ulcerative colitis (UC) remains unknown. The present study was conducted to 
      verify whether the anti-inflammation effect of baicalin on experimental colitis 
      is via inhibiting TLR4/NF-kappaB pathway activation. METHODS: The inflammatory 
      response in RAW264.7 cells was induced by LPS and in rats by intrarectal 
      administration of TNBS. Western blot analysis was carried out to examine 
      toll-like receptor 4 (TLR4), NF-kappaB, p-NF-kappaB p65, IkappaB and p-IkappaB protein 
      expressions in cells. Furthermore, intercellular adhesion molecule-1 (ICAM-1), 
      monocyte chemoattractant protein-1 (MCP-1), cyclo-oxygenase-2 (Cox-2), tumor 
      necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta and IL-6 levels in cell 
      supernatant and rat serum were detected by appropriate kits. An 
      immunohistochemical assay was applied to examine TNF-alpha and IL-1beta protein 
      expression in colon tissues and TLR4 and p-NF-kappaB p65 protein expressions in 
      RAW264.7 cells. RESULTS: Baicalin ameliorates the considered inflammatory 
      symptoms of induced colitis. It could also down-regulate pro-inflammatory 
      mediators in the colon mucosa. The decline in the production of pro-inflammatory 
      cytokines was correlated with the decrease in mucosal TLR4 protein expression. 
      The expression of p-NF-kappaB p65 protein was significantly decreased, which 
      correlated with a similar decrease in p-IkappaB protein. Consistent with the in vivo 
      results, baicalin blocked LPS-stimulated nuclear translocation of p-NF-kappaB p65 in 
      mouse macrophage RAW264.7 cells. CONCLUSIONS: The present study indicates for the 
      first time that the mechanism for baicalin on abrogating experimental colitis was 
      targeted inhibition of the TLR4/NF-kappaB pathway activation.
CI  - Copyright (c) 2014. Published by Elsevier B.V.
FAU - Cui, Li
AU  - Cui L
AD  - Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu 
      Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China.
FAU - Feng, Liang
AU  - Feng L
AD  - Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu 
      Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China.
FAU - Zhang, Zhen Hai
AU  - Zhang ZH
AD  - Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu 
      Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China.
FAU - Jia, Xiao Bin
AU  - Jia XB
AD  - Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Jiangsu 
      Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140918
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Flavonoids)
RN  - 0 (NF-kappa B)
RN  - 0 (Toll-Like Receptor 4)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 347Q89U4M5 (baicalin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*administration & dosage
MH  - Cell Line
MH  - Chemokine CCL2/metabolism
MH  - Colitis, Ulcerative/*drug therapy/immunology
MH  - Colon/*drug effects/immunology
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Flavonoids/*administration & dosage
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Male
MH  - Mice
MH  - NF-kappa B/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - Toll-Like Receptor 4/*metabolism
OTO - NOTNLM
OT  - Baicalin
OT  - NF-kappaB
OT  - Pathway
OT  - TLR4
OT  - Ulcerative colitis
EDAT- 2014/09/23 06:00
MHDA- 2015/08/26 06:00
CRDT- 2014/09/21 06:00
PHST- 2014/08/06 00:00 [received]
PHST- 2014/08/19 00:00 [revised]
PHST- 2014/09/05 00:00 [accepted]
PHST- 2014/09/21 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2015/08/26 06:00 [medline]
AID - S1567-5769(14)00346-4 [pii]
AID - 10.1016/j.intimp.2014.09.005 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2014 Nov;23(1):294-303. doi: 10.1016/j.intimp.2014.09.005. 
      Epub 2014 Sep 18.