PMID- 25239864
OWN - NLM
STAT- MEDLINE
DCOM- 20150305
LR  - 20141203
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 63
IP  - 2
DP  - 2015 Feb
TI  - Tat-biliverdin reductase A inhibits inflammatory response by regulation of MAPK 
      and NF-kappaB pathways in Raw 264.7 cells and edema mouse model.
PG  - 355-66
LID - S0161-5890(14)00236-3 [pii]
LID - 10.1016/j.molimm.2014.09.003 [doi]
AB  - Reactive oxygen species (ROS) accumulation induces oxidative stress and cell 
      damage, which then activates several signaling pathways and triggers inflammatory 
      response. Biliverdin is a natural product of heme metabolism which is converted 
      to bilirubin by the enzyme biliverdin reductase A (BLVRA) which also plays a role 
      in antioxidant activity via the ROS scavenging activity of bilirubin. In this 
      study, we examined the anti-inflammatory and anti-apoptotic effects of Tat-BLVRA 
      protein on lipopolysaccharide (LPS)-induced inflammation in Raw 264.7 macrophage 
      cells. Transduction of Tat-BLVRA protein into Raw 264.7 cells and mice ear tissue 
      was tested by Western blot analysis and immunohistochemical analysis. Tat-BLVRA 
      protein was effective in inhibiting mitogen activated protein kinases (MAPKs), 
      Akt and NF-kappaB activation, intracellular ROS production and DNA fragmentation. 
      Also, Tat-BLVRA protein significantly inhibited the expression of cytokines, 
      COX-2, and iNOS. In a 12-O-tetradecanoylphobol 13-acetate (TPA)-induced mouse 
      model, mice ears treated with Tat-BLVRA protein showed decreased ear thickness 
      and weight, as well as inhibited MAPKs activation and cytokine expression. Thus 
      we suggested that Tat-BLVRA protein may provide an effective therapeutic agent 
      for inflammatory skin diseases.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Kim, Hye Ri
AU  - Kim HR
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Kim, Dae Won
AU  - Kim DW
AD  - Department of Biochemistry and Molecular Biology, Research Institute of Oral 
      Sciences, College of Dentistry, Kangnung-Wonju National University, Gangneung 
      210-702, South Korea.
FAU - Jo, Hyo Sang
AU  - Jo HS
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Cho, Su Bin
AU  - Cho SB
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Park, Jung Hwan
AU  - Park JH
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Lee, Chi Hern
AU  - Lee CH
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Choi, Yeon Joo
AU  - Choi YJ
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Yeo, Eun Ji
AU  - Yeo EJ
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Park, Sung Yeon
AU  - Park SY
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Kim, Seung Tae
AU  - Kim ST
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Yu, Yeon Hee
AU  - Yu YH
AD  - Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan-Si 
      330-090, South Korea.
FAU - Kim, Duk-Soo
AU  - Kim DS
AD  - Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan-Si 
      330-090, South Korea.
FAU - Kim, Hyun Ah
AU  - Kim HA
AD  - Division of Rheumatology, Department of Internal Medicine, Hallym University 
      Sacred Heart Hospital, Pyongchon, Kyunggido 431-070, South Korea.
FAU - Cho, Sung-Woo
AU  - Cho SW
AD  - Department of Biochemistry and Molecular Biology, University of Ulsan College of 
      Medicine, Seoul 138-736, South Korea.
FAU - Han, Kyu Hyung
AU  - Han KH
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Park, Jinseu
AU  - Park J
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea.
FAU - Eum, Won Sik
AU  - Eum WS
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea. Electronic 
      address: wseum@hallym.ac.kr.
FAU - Choi, Soo Young
AU  - Choi SY
AD  - Department of Biomedical Science and Research Institute of Bioscience and 
      Biotechnology, Hallym University, Chunchon 200-702, South Korea. Electronic 
      address: sychoi@hallym.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140917
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
RN  - EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors)
RN  - EC 1.3.1.24 (biliverdin reductase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Disease Models, Animal
MH  - Edema/pathology/*therapy
MH  - Humans
MH  - Inflammation/enzymology/*pathology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/drug effects/enzymology/*pathology
MH  - Male
MH  - Mice, Inbred ICR
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Oxidative Stress/drug effects
MH  - Oxidoreductases Acting on CH-CH Group Donors/*genetics/*therapeutic use
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Recombinant Fusion Proteins/isolation & purification
MH  - Signal Transduction
MH  - Tetradecanoylphorbol Acetate
MH  - Transduction, Genetic
MH  - tat Gene Products, Human Immunodeficiency Virus/metabolism/*therapeutic use
OTO - NOTNLM
OT  - Cytokine
OT  - Inflammation
OT  - MAPK
OT  - Protein therapy
OT  - ROS
OT  - Tat-BLVRA
EDAT- 2014/09/23 06:00
MHDA- 2015/03/07 06:00
CRDT- 2014/09/21 06:00
PHST- 2014/07/15 00:00 [received]
PHST- 2014/08/31 00:00 [revised]
PHST- 2014/09/01 00:00 [accepted]
PHST- 2014/09/21 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2015/03/07 06:00 [medline]
AID - S0161-5890(14)00236-3 [pii]
AID - 10.1016/j.molimm.2014.09.003 [doi]
PST - ppublish
SO  - Mol Immunol. 2015 Feb;63(2):355-66. doi: 10.1016/j.molimm.2014.09.003. Epub 2014 
      Sep 17.