PMID- 25240243
OWN - NLM
STAT- MEDLINE
DCOM- 20160607
LR  - 20160125
IS  - 1097-6809 (Electronic)
IS  - 0741-5214 (Linking)
VI  - 63
IP  - 2
DP  - 2016 Feb
TI  - Inhibitory effects of mesenchymal stem cells in intimal hyperplasia after balloon 
      angioplasty.
PG  - 510-7
LID - S0741-5214(14)01624-3 [pii]
LID - 10.1016/j.jvs.2014.08.058 [doi]
AB  - OBJECTIVE: Intimal hyperplasia is a major cause of restenosis after arterial 
      bypass and balloon angioplasty. Induction of rapid re-endothelialization has been 
      proposed to reduce intimal hyperplasia. The aim of this study was to evaluate the 
      inhibitory effect of mesenchymal stem cells on intimal hyperplasia. METHODS: Male 
      New Zealand white rabbits were fed 1% cholesterol diets from 1 week before 
      balloon angioplasty to the day of harvest. After dissection of rabbit carotid 
      arteries, balloon angioplasty was performed with a 2F Fogarty embolectomy 
      catheter. The injured carotid artery was coated with a mixture of 7 x 10(6) human 
      umbilical cord mesenchymal stem cells (HUC-MSCs) and fibrin matrix. The carotid 
      arteries were harvested 2, 4, and 8 weeks thereafter, and immunofluorescent 
      staining and quantitative real-time polymerase chain reaction analysis were 
      performed. RESULTS: The intima/media ratio was significantly reduced in the group 
      treated with HUC-MSCs compared with the nontreated group (Student t-tests, *P < 
      .05). The area of re-endothelialization was significantly higher (Student 
      t-tests, *P < .05) in the group treated with HUC-MSCs than in the nontreated 
      group. Expression of angiogenic genes such as vascular endothelial growth factor, 
      platelet-derived growth factor, kinase insert domain receptor 1, angiopoietin 1, 
      and angio-associated migratory cell protein was increased (analysis of variance, 
      P < .05) in the group treated with HUC-MSCs relative to the nontreated group. 
      CONCLUSIONS: Our study showed that HUC-MSCs reduce the formation of intimal 
      hyperplasia through rapid re-endothelialization. This result might be applied to 
      development of stem cell-coated stents as well as to development of a stem 
      cell-containing sheet coat for inhibition of intimal hyperplasia after 
      angioplasty or surgery.
CI  - Copyright (c) 2016 Society for Vascular Surgery. Published by Elsevier Inc. All 
      rights reserved.
FAU - Kim, Ae-Kyeong
AU  - Kim AK
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Kim, Min-Hee
AU  - Kim MH
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Kim, Do-Hyung
AU  - Kim DH
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Go, Ha-Nl
AU  - Go HN
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Cho, Seung-Woo
AU  - Cho SW
AD  - Department of Biotechnology, Yonsei University, Seoul, Korea.
FAU - Um, Soong Ho
AU  - Um SH
AD  - School of Chemical Engineering and SKKU Advanced Institute of Nanotechnology, 
      Sungkyunkwan University, Seoul, Korea.
FAU - Kim, Dong-Ik
AU  - Kim DI
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea. Electronic address: dikim@skku.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140918
PL  - United States
TA  - J Vasc Surg
JT  - Journal of vascular surgery
JID - 8407742
RN  - 0 (Angiogenic Proteins)
SB  - IM
MH  - Angiogenic Proteins/genetics/metabolism
MH  - *Angioplasty, Balloon
MH  - Animals
MH  - Carotid Arteries/metabolism/*pathology
MH  - Carotid Artery Injuries/etiology/genetics/metabolism/pathology/*surgery
MH  - Diet, High-Fat
MH  - Disease Models, Animal
MH  - Gene Expression Regulation
MH  - Humans
MH  - Hyperplasia
MH  - Macrophages/metabolism/pathology
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Neointima
MH  - Rabbits
MH  - Re-Epithelialization
MH  - Time Factors
MH  - Vascular Remodeling
EDAT- 2014/09/23 06:00
MHDA- 2016/06/09 06:00
CRDT- 2014/09/22 06:00
PHST- 2014/05/16 00:00 [received]
PHST- 2014/08/05 00:00 [accepted]
PHST- 2014/09/22 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2016/06/09 06:00 [medline]
AID - S0741-5214(14)01624-3 [pii]
AID - 10.1016/j.jvs.2014.08.058 [doi]
PST - ppublish
SO  - J Vasc Surg. 2016 Feb;63(2):510-7. doi: 10.1016/j.jvs.2014.08.058. Epub 2014 Sep 
      18.