PMID- 25240886
OWN - NLM
STAT- MEDLINE
DCOM- 20150623
LR  - 20140922
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1206
DP  - 2015
TI  - Identification and validation of miRNA target sites within nontraditional miRNA 
      targets.
PG  - 53-67
LID - 10.1007/978-1-4939-1369-5_5 [doi]
AB  - miRNAs are endogenous small RNAs that regulate gene expression through 
      recognition of complementary RNA sequences. While miRNAs have generally been 
      understood to repress gene expression posttranscriptionally through recognition 
      of 3'-untranslated regions (3'-UTRs) of mRNA transcripts, they have the potential 
      to target additional classes of RNAs. Understanding the expanding pool of 
      potential miRNA targets has been hindered by the lack of tools for predicting 
      target sites within these RNAs. Here, the principles for developing computational 
      algorithms for identifying putative miRNA target sites outside of mRNA are 
      discussed. Laboratory techniques for validating computational miRNA target 
      predictions are described.
FAU - Younger, Scott T
AU  - Younger ST
AD  - Departments of Pharmacology and Biochemistry, UT Southwestern Medical Center, 
      5323 Harry Hines Blvd., Dallas, TX, 75390-9041, USA.
FAU - Corey, David R
AU  - Corey DR
LA  - eng
GR  - 77253/PHS HHS/United States
GR  - GM07062/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MicroRNAs)
RN  - 0 (Oligonucleotides)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - 3' Untranslated Regions
MH  - *Algorithms
MH  - Databases, Genetic
MH  - MicroRNAs/antagonists & inhibitors/*genetics/*metabolism
MH  - Oligonucleotides/genetics
MH  - RNA, Small Interfering
MH  - Reproducibility of Results
EDAT- 2014/09/23 06:00
MHDA- 2015/06/24 06:00
CRDT- 2014/09/22 06:00
PHST- 2014/09/22 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2015/06/24 06:00 [medline]
AID - 10.1007/978-1-4939-1369-5_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1206:53-67. doi: 10.1007/978-1-4939-1369-5_5.