PMID- 25240949
OWN - NLM
STAT- MEDLINE
DCOM- 20150911
LR  - 20181202
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 119
DP  - 2015 Jan
TI  - Effects of fluoxetine on behavior, antioxidant enzyme systems, and 
      multixenobiotic resistance in the Asian clam Corbicula fluminea.
PG  - 856-862
LID - S0045-6535(14)01035-2 [pii]
LID - 10.1016/j.chemosphere.2014.08.062 [doi]
AB  - Fluoxetine (FLX), a selective serotonin reuptake inhibitor, is widespread in 
      aquatic environments. Despite its reported effects on behavior and reproduction 
      in aquatic species, little is known about the effects of FLX on cellular 
      detoxification and defense system in bivalves. Here, the adult Asian clam 
      (Corbicula fluminea) was exposed to 0.5, 5, and 50 mug L(-1) FLX for 30 d. 
      Siphoning behavior was inhibited by treatment with 50 mug L(-1) FLX. Additionally, 
      superoxide dismutase (SOD) activity in the gills and digestive glands 
      significantly decreased (p<0.05) with 5 and 50 mug L(-1) FLX treatments, whereas 
      catalase (CAT) activity and malondialdehyde (MDA) content markedly increased 
      (p<0.05). Moreover, transcription of thioredoxin reductase (TR), glutathione 
      peroxidase (GPx) and Glutathione S-transferase pi class (GSTpi) was significantly 
      upregulated (p<0.05), whereas glutathione reductase (GR) was markedly 
      downregulated (p<0.05). These findings suggest that FLX affects behavior and 
      induces oxidative stress in C. fluminea. The downregulation of ATP-binding 
      cassette (ABC) transportor genes (ABCB1, ABCC1 and ABCG2) transporter genes 
      indicated that FLX might suppress the multixenobiotic resistance (MXR) system in 
      C. fluminea. Our results provide new insights into the adverse effects of FLX on 
      the cellular detoxification and MXR system of C. fluminea.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Chen, Huihui
AU  - Chen H
AD  - State Key Laboratory of Environmental Aquatic Chemistry, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, People's 
      Republic of China.
FAU - Zha, Jinmiao
AU  - Zha J
AD  - State Key Laboratory of Environmental Aquatic Chemistry, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, People's 
      Republic of China. Electronic address: jmzha@rcees.ac.cn.
FAU - Yuan, Lilai
AU  - Yuan L
AD  - State Key Laboratory of Environmental Aquatic Chemistry, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, People's 
      Republic of China.
FAU - Wang, Zijian
AU  - Wang Z
AD  - State Key Laboratory of Environmental Aquatic Chemistry, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, People's 
      Republic of China. Electronic address: wangzj@rcees.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140919
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Antioxidants)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Water Pollutants, Chemical)
RN  - 01K63SUP8D (Fluoxetine)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.8.1.7 (Glutathione Reductase)
RN  - EC 1.8.1.9 (Thioredoxin-Disulfide Reductase)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Behavior, Animal/*drug effects
MH  - Catalase/metabolism
MH  - China
MH  - Corbicula/*drug effects/genetics
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Dose-Response Relationship, Drug
MH  - Fluoxetine/metabolism/*toxicity
MH  - Gills/metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - Glutathione Reductase/metabolism
MH  - Malondialdehyde/metabolism
MH  - Oxidative Stress/drug effects
MH  - Real-Time Polymerase Chain Reaction
MH  - Superoxide Dismutase/metabolism
MH  - Thioredoxin-Disulfide Reductase/metabolism
MH  - Water Pollutants, Chemical/analysis/*toxicity
OTO - NOTNLM
OT  - Antioxidant enzymes
OT  - C. fluminea
OT  - Fluoxetine
OT  - Multixenobiotic resistance
OT  - Siphoning behavior
EDAT- 2014/09/23 06:00
MHDA- 2015/09/12 06:00
CRDT- 2014/09/22 06:00
PHST- 2014/04/04 00:00 [received]
PHST- 2014/08/19 00:00 [revised]
PHST- 2014/08/21 00:00 [accepted]
PHST- 2014/09/22 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
AID - S0045-6535(14)01035-2 [pii]
AID - 10.1016/j.chemosphere.2014.08.062 [doi]
PST - ppublish
SO  - Chemosphere. 2015 Jan;119:856-862. doi: 10.1016/j.chemosphere.2014.08.062. Epub 
      2014 Sep 19.