PMID- 25242211
OWN - NLM
STAT- MEDLINE
DCOM- 20141217
LR  - 20220311
IS  - 1090-2430 (Electronic)
IS  - 0014-4886 (Linking)
VI  - 261
DP  - 2014 Nov
TI  - Over-expression of P2X7 receptors in spinal glial cells contributes to the 
      development of chronic postsurgical pain induced by skin/muscle incision and 
      retraction (SMIR) in rats.
PG  - 836-43
LID - S0014-4886(14)00290-8 [pii]
LID - 10.1016/j.expneurol.2014.09.007 [doi]
AB  - Many patients suffer from chronic postsurgical pain (CPSP) following surgery, and 
      the underlying mechanisms are poorly understood. In the present work, with use of 
      the skin/muscle incision and retraction (SMIR) model, the role of P2X7 receptors 
      (P2X7Rs) in spinal glial cells in the development of CPSP was evaluated. 
      Consistent with previous reports, we found that SMIR decreased the ipsilateral 
      50% paw withdrawal threshold (PWT), lasting for at least 2weeks. No injury was 
      done to L3 dorsal root ganglia (DRG) neurons and no axonal or Schwann cell damage 
      at the retraction site in the saphenous nerve was observed 7days after SMIR. The 
      results of immunofluorescence showed that both microglia and astrocytes were 
      activated in the spinal dorsal horn following SMIR. In addition, both P2X7Rs and 
      tumor necrosis factor-alpha (TNF-alpha) were up-regulated following SMIR. Double 
      immunofluorescence staining revealed that the up-regulated P2X7R immunoreactivity 
      was mainly located in microglia, and to a lesser extent in astrocytes, but not in 
      neurons. Intrathecal delivery of specific P2X7R antagonist BBG (10muM in 10mul 
      volume) or A438079 (10muM in 10mul volume), started 30min before the surgery and 
      once daily thereafter for 7days, prevented the mechanical allodynia. Intrathecal 
      injection of BBG inhibited the activation of microglia and astrocytes, and the 
      up-regulation of TNF-alpha induced by SMIR. These data suggest that P2X7Rs in the 
      spinal dorsal horn might mediate the development of CPSP via activation of glial 
      cells and up-regulation of TNF-alpha.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Ying, Yan-Lu
AU  - Ying YL
AD  - Department of Anesthesiology, Guangzhou First People's Hospital, Guangzhou 
      Medical University, 602 Renminbei Rd., Guangzhou 510180, China.
FAU - Wei, Xu-Hong
AU  - Wei XH
AD  - Department of Physiology and Pain Research Center, Zhongshan School of Medicine, 
      Sun Yat-Sen University, 74 Zhongshan Rd. 2, Guangzhou 510080, China.
FAU - Xu, Xue-Bing
AU  - Xu XB
AD  - Department of Anesthesiology, The University of Hong Kong-Shenzhen Hospital, 
      Haiyuan 1st Road, Futian District, Shenzhen 518053, China. Electronic address: 
      xuxuebing@hotmail.com.
FAU - She, Shou-Zhang
AU  - She SZ
AD  - Department of Anesthesiology, Guangzhou First People's Hospital, Guangzhou 
      Medical University, 602 Renminbei Rd., Guangzhou 510180, China.
FAU - Zhou, Li-Jun
AU  - Zhou LJ
AD  - Department of Physiology and Pain Research Center, Zhongshan School of Medicine, 
      Sun Yat-Sen University, 74 Zhongshan Rd. 2, Guangzhou 510080, China.
FAU - Lv, Jing
AU  - Lv J
AD  - Department of Anesthesiology, Guangzhou First People's Hospital, Guangzhou 
      Medical University, 602 Renminbei Rd., Guangzhou 510180, China.
FAU - Li, Dai
AU  - Li D
AD  - Department of Physiology and Pain Research Center, Zhongshan School of Medicine, 
      Sun Yat-Sen University, 74 Zhongshan Rd. 2, Guangzhou 510080, China.
FAU - Zheng, Bin
AU  - Zheng B
AD  - Department of Anesthesiology, Guangzhou First People's Hospital, Guangzhou 
      Medical University, 602 Renminbei Rd., Guangzhou 510180, China.
FAU - Liu, Xian-Guo
AU  - Liu XG
AD  - Department of Physiology and Pain Research Center, Zhongshan School of Medicine, 
      Sun Yat-Sen University, 74 Zhongshan Rd. 2, Guangzhou 510080, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140919
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (3-(5-(2,3-dichlorophenyl)-1H-tetrazol-1-yl)methylpyridine)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Purinergic P2X Receptor Antagonists)
RN  - 0 (Pyridines)
RN  - 0 (Receptors, Purinergic P2X7)
RN  - 0 (Tetrazoles)
SB  - IM
MH  - Animals
MH  - Dermatologic Surgical Procedures/adverse effects
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Functional Laterality
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Muscles/surgery
MH  - Neuroglia/*metabolism/ultrastructure
MH  - Pain, Postoperative/drug therapy/etiology/*pathology
MH  - Purinergic P2X Receptor Antagonists/therapeutic use
MH  - Pyridines/therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Purinergic P2X7/*metabolism
MH  - Skin
MH  - Spinal Cord/*pathology
MH  - Tetrazoles/therapeutic use
MH  - Time Factors
MH  - Up-Regulation/drug effects/physiology
OTO - NOTNLM
OT  - Chronic postsurgical pain
OT  - Microglia
OT  - P2X7 receptor
OT  - Tumor necrosis factor-alpha
EDAT- 2014/09/23 06:00
MHDA- 2014/12/18 06:00
CRDT- 2014/09/23 06:00
PHST- 2014/04/26 00:00 [received]
PHST- 2014/08/10 00:00 [revised]
PHST- 2014/09/05 00:00 [accepted]
PHST- 2014/09/23 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2014/12/18 06:00 [medline]
AID - S0014-4886(14)00290-8 [pii]
AID - 10.1016/j.expneurol.2014.09.007 [doi]
PST - ppublish
SO  - Exp Neurol. 2014 Nov;261:836-43. doi: 10.1016/j.expneurol.2014.09.007. Epub 2014 
      Sep 19.