PMID- 25242642
OWN - NLM
STAT- MEDLINE
DCOM- 20150812
LR  - 20220310
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 280
DP  - 2014 Nov 7
TI  - Human dental pulp stem cells respond to cues from the rat retina and 
      differentiate to express the retinal neuronal marker rhodopsin.
PG  - 142-55
LID - S0306-4522(14)00765-9 [pii]
LID - 10.1016/j.neuroscience.2014.09.023 [doi]
AB  - Human adult dental pulp stem cells (DPSCs) are self-renewing stem cells that 
      originate from the neural crest during development and remain within the dental 
      pulp niche through adulthood. Due to their multi-lineage differentiation 
      potential and their relative ease of access they represent an exciting 
      alternative for autologous stem cell-based therapies in neurodegenerative 
      diseases. In animal models, DPSCs transplanted into the brain differentiate into 
      functional neurons or astrocytes in response to local environmental cues that 
      appear to influence the fate of the surviving cells. Here we tested the 
      hypothesis that DPSCs might be able to respond to factors present in the retina 
      enabling the regenerative potential of these cells. We evaluated the response of 
      DPSCs to conditioned media from organotypic explants from control and chemically 
      damaged rat retinas. To evaluate cell differentiation, we analyzed the expression 
      of glial fibrillary acidic protein (GFAP), early neuronal and retinal markers 
      (polysialic acid-neural cell adhesion molecule (PSA-NCAM); Pax6; Ascl1; NeuroD1) 
      and the late photoreceptor marker rhodopsin, by immunofluorescence and reverse 
      transcription polymerase chain reaction (RT-PCR). Exposure of DPSC cultures to 
      conditioned media from control retinas induced a 39% reduction on the number of 
      DPSCs that expressed GFAP; the expression of Pax6, Ascl1, PSA-NCAM or NeuroD1 was 
      undetectable or did not change significantly. Expression of rhodopsin was not 
      detectable in control or after exposure of the cultures with retinal conditioned 
      media. By contrast, 44% of DPSCs exposed to conditioned media from damaged 
      retinas were immunopositive to this protein. This response could not be 
      reproduced when conditioned media from Muller-enriched primary cultures was used. 
      Finally, quantitative RT-PCR was performed to compare the relative expression of 
      glial cell-derived neurotrophic factor (GDNF), nerve growth factor (NGF), ciliary 
      neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) in DPSC 
      co-cultured with retinal organotypic explants, where BDNF mRNA expression was 
      significantly upregulated in retinal-exposed cultures. Our data demonstrate that 
      DPSC cultures respond to cues from the rat retina and differentiate to express 
      retinal neuronal markers.
CI  - Copyright (c) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Bray, A F
AU  - Bray AF
AD  - Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, 
      Mexico D.F., Mexico; Departamento de Farmacobiologia, Centro de Investigacion y 
      de Estudios Avanzados del IPN, Mexico D.F., Mexico.
FAU - Cevallos, R R
AU  - Cevallos RR
AD  - Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, 
      Mexico D.F., Mexico.
FAU - Gazarian, K
AU  - Gazarian K
AD  - Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, 
      Mexico D.F., Mexico.
FAU - Lamas, M
AU  - Lamas M
AD  - Departamento de Farmacobiologia, Centro de Investigacion y de Estudios Avanzados 
      del IPN, Mexico D.F., Mexico. Electronic address: mlamas@cinvestav.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140919
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neural Cell Adhesion Molecule L1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sialic Acids)
RN  - 0 (polysialyl neural cell adhesion molecule)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - 9009-81-8 (Rhodopsin)
SB  - IM
MH  - Adult
MH  - Adult Stem Cells/cytology/*physiology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Culture Techniques
MH  - Cell Differentiation/*physiology
MH  - Coculture Techniques
MH  - Culture Media, Conditioned
MH  - Dental Pulp/cytology/*physiology
MH  - Ependymoglial Cells/metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Humans
MH  - Nerve Growth Factors/metabolism
MH  - Neural Cell Adhesion Molecule L1/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats, Long-Evans
MH  - Retina/injuries/*metabolism
MH  - Rhodopsin/*metabolism
MH  - Sialic Acids/metabolism
MH  - Tissue Culture Techniques
MH  - Young Adult
OTO - NOTNLM
OT  - Muller glia
OT  - dental pulp stem cells
OT  - neuronal differentiation
OT  - retina
EDAT- 2014/09/23 06:00
MHDA- 2015/08/13 06:00
CRDT- 2014/09/23 06:00
PHST- 2014/06/24 00:00 [received]
PHST- 2014/09/05 00:00 [revised]
PHST- 2014/09/10 00:00 [accepted]
PHST- 2014/09/23 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2015/08/13 06:00 [medline]
AID - S0306-4522(14)00765-9 [pii]
AID - 10.1016/j.neuroscience.2014.09.023 [doi]
PST - ppublish
SO  - Neuroscience. 2014 Nov 7;280:142-55. doi: 10.1016/j.neuroscience.2014.09.023. 
      Epub 2014 Sep 19.