PMID- 25242947
OWN - NLM
STAT- MEDLINE
DCOM- 20150528
LR  - 20211021
IS  - 1678-4405 (Electronic)
IS  - 1517-8382 (Print)
IS  - 1517-8382 (Linking)
VI  - 45
IP  - 2
DP  - 2014
TI  - Bioinformatics based structural characterization of glucose dehydrogenase (gdh) 
      gene and growth promoting activity of Leclercia sp. QAU-66.
PG  - 603-11
AB  - Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group 
      that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose 
      as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated 
      from rhizosphere of Vigna mungo, was characterized for phosphate solubilization 
      and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain 
      QAU-66 had ability to solubilize phosphorus and significantly (p </= 0.05) promoted 
      the shoot and root lengths of Phaseolus vulgaris. The structural determination of 
      GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, 
      I-TASSER and COFACTOR. These tools predicted the structural based functional 
      homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 
      is one of the main factor that involved in plant growth promotion and provides a 
      solid background for further research in plant growth promoting activities.
FAU - Naveed, Muhammad
AU  - Naveed M
AD  - Plant Genomics Lab Department of Plant Sciences Quaid-i-Azam University Islamabad 
      Pakistan Plant Genomics Lab, Department of Plant Sciences, Quaid-i-Azam 
      University, Islamabad, Pakistan.
FAU - Ahmed, Iftikhar
AU  - Ahmed I
AD  - National Institute for Genomics & Advanced Biotechnology National Agricultural 
      Research Centre Islamabad Pakistan National Institute for Genomics & Advanced 
      Biotechnology, National Agricultural Research Centre, Islamabad, Pakistan.
FAU - Khalid, Nauman
AU  - Khalid N
AD  - Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo 
      Japan Graduate School of Agricultural and Life Sciences, The University of Tokyo, 
      Tokyo, Japan.
FAU - Mumtaz, Abdul Samad
AU  - Mumtaz AS
AD  - Plant Genomics Lab Department of Plant Sciences Quaid-i-Azam University Islamabad 
      Pakistan Plant Genomics Lab, Department of Plant Sciences, Quaid-i-Azam 
      University, Islamabad, Pakistan.
LA  - eng
SI  - GENBANK/KC679993
SI  - GENBANK/KF156751
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140829
PL  - Brazil
TA  - Braz J Microbiol
JT  - Brazilian journal of microbiology : [publication of the Brazilian Society for 
      Microbiology]
JID - 101095924
RN  - 0 (DNA, Bacterial)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Quinones)
RN  - 27YLU75U4W (Phosphorus)
RN  - EC 1.1.1.47 (Glucose 1-Dehydrogenase)
SB  - IM
MH  - Cluster Analysis
MH  - Computational Biology
MH  - Cytosol/chemistry
MH  - DNA, Bacterial/chemistry/genetics
MH  - Enterobacteriaceae/*enzymology/genetics/isolation & purification/*physiology
MH  - Glucose 1-Dehydrogenase/chemistry/*genetics
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - *Nerve Growth Factors
MH  - Phaseolus/*growth & development/*microbiology
MH  - Phosphorus/metabolism
MH  - Phylogeny
MH  - Plant Roots/growth & development
MH  - Plant Shoots/growth & development
MH  - Protein Conformation
MH  - Protein Structure, Tertiary
MH  - Quinones/analysis
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
PMC - PMC4166288
OTO - NOTNLM
OT  - Leclercia sp.
OT  - PGPR
OT  - bioinformatics
OT  - glucose dehydrogenase
OT  - pyrroloquinoline quinone
EDAT- 2014/09/23 06:00
MHDA- 2015/05/29 06:00
PMCR- 2014/09/29
CRDT- 2014/09/23 06:00
PHST- 2013/06/09 00:00 [received]
PHST- 2013/09/09 00:00 [accepted]
PHST- 2014/09/23 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2015/05/29 06:00 [medline]
PHST- 2014/09/29 00:00 [pmc-release]
AID - bjm-45-603 [pii]
AID - 10.1590/s1517-83822014000200031 [doi]
PST - epublish
SO  - Braz J Microbiol. 2014 Aug 29;45(2):603-11. doi: 10.1590/s1517-83822014000200031. 
      eCollection 2014.