PMID- 25243114
OWN - NLM
STAT- MEDLINE
DCOM- 20160515
LR  - 20211021
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2014
DP  - 2014
TI  - Characterisation of potential antidiabetic-related proteins from Pleurotus 
      pulmonarius (Fr.) Quel. (grey oyster mushroom) by MALDI-TOF/TOF mass 
      spectrometry.
PG  - 131607
LID - 10.1155/2014/131607 [doi]
LID - 131607
AB  - Pleurotus pulmonarius has been reported to have a potent remedial effect on 
      diabetic property and considered to be an alternative for type 2 diabetes 
      mellitus treatment. This study aimed to investigate the antidiabetic properties 
      of ammonium sulphate precipitated protein fractions from P. pulmonarius 
      basidiocarps. Preliminary results demonstrated that 30% (NH4)2SO4 precipitated 
      fraction (F30) inhibited Saccharomyces cerevisiae alpha-glucosidase activity 
      (24.18%), and 100% (NH4)2SO4 precipitated fraction (F100) inhibited porcine 
      pancreatic alpha-amylase activity (41.80%). Following RP-HPLC purification, peak 3 
      from F30 fraction demonstrated inhibition towards alpha-glucosidase at the same time 
      with meagre inhibition towards alpha-amylase activity. Characterisation of proteins 
      using MALDI-TOF/TOF MS demonstrated the presence of four different proteins, 
      which could be implicated in the regulation of blood glucose level via various 
      mechanisms. Therefore, this study revealed the presence of four 
      antidiabetic-related proteins which are profilin-like protein, 
      glyceraldehyde-3-phosphate dehydrogenase-like protein, trehalose 
      phosphorylase-like (TP-like) protein, and catalase-like protein. Hence, P. 
      pulmonarius basidiocarps have high potential in lowering blood glucose level, 
      reducing insulin resistance and vascular complications.
FAU - Wahab, Nurul Azwa Abd
AU  - Wahab NA
AD  - Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, 
      University of Malaya, 50603 Kuala Lumpur, Malaysia.
FAU - Abdullah, Noorlidah
AU  - Abdullah N
AD  - Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, 
      University of Malaya, 50603 Kuala Lumpur, Malaysia.
FAU - Aminudin, Norhaniza
AU  - Aminudin N
AD  - Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, 
      University of Malaya, 50603 Kuala Lumpur, Malaysia ; University of Malaya Centre 
      for Proteomics Research, University of Malaya, 50603 Kuala Lumpur, Malaysia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140828
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Fungal Proteins)
RN  - 0 (Hypoglycemic Agents)
RN  - EC 3.2.1.1 (alpha-Amylases)
RN  - EC 3.2.1.20 (alpha-Glucosidases)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Reverse-Phase
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fungal Proteins/*pharmacology
MH  - Hypoglycemic Agents/*pharmacology
MH  - Pleurotus/*chemistry
MH  - Silver Staining
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - alpha-Amylases/metabolism
MH  - alpha-Glucosidases/metabolism
PMC - PMC4163432
EDAT- 2014/09/23 06:00
MHDA- 2016/05/18 06:00
PMCR- 2014/08/28
CRDT- 2014/09/23 06:00
PHST- 2014/02/09 00:00 [received]
PHST- 2014/06/04 00:00 [revised]
PHST- 2014/07/22 00:00 [accepted]
PHST- 2014/09/23 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2016/05/18 06:00 [medline]
PHST- 2014/08/28 00:00 [pmc-release]
AID - 10.1155/2014/131607 [doi]
PST - ppublish
SO  - Biomed Res Int. 2014;2014:131607. doi: 10.1155/2014/131607. Epub 2014 Aug 28.