PMID- 25243135
OWN - NLM
STAT- MEDLINE
DCOM- 20160515
LR  - 20211021
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2014
DP  - 2014
TI  - BDNF increases survival and neuronal differentiation of human neural precursor 
      cells cotransplanted with a nanofiber gel to the auditory nerve in a rat model of 
      neuronal damage.
PG  - 356415
LID - 10.1155/2014/356415 [doi]
LID - 356415
AB  - OBJECTIVES: To study possible nerve regeneration of a damaged auditory nerve by 
      the use of stem cell transplantation. METHODS: We transplanted HNPCs to the rat 
      AN trunk by the internal auditory meatus (IAM). Furthermore, we studied if 
      addition of BDNF affects survival and phenotypic differentiation of the grafted 
      HNPCs. A bioactive nanofiber gel (PA gel), in selected groups mixed with BDNF, 
      was applied close to the implanted cells. Before transplantation, all rats had 
      been deafened by a round window niche application of beta-bungarotoxin. This 
      neurotoxin causes a selective toxic destruction of the AN while keeping the hair 
      cells intact. RESULTS: Overall, HNPCs survived well for up to six weeks in all 
      groups. However, transplants receiving the BDNF-containing PA gel demonstrated 
      significantly higher numbers of HNPCs and neuronal differentiation. At six weeks, 
      a majority of the HNPCs had migrated into the brain stem and differentiated. 
      Differentiated human cells as well as neurites were observed in the vicinity of 
      the cochlear nucleus. CONCLUSION: Our results indicate that human neural 
      precursor cells (HNPC) integration with host tissue benefits from additional 
      brain derived neurotrophic factor (BDNF) treatment and that these cells appear to 
      be good candidates for further regenerative studies on the auditory nerve (AN).
FAU - Jiao, Yu
AU  - Jiao Y
AD  - Center for Hearing and Communication Research, Karolinska University Hospital, 
      171 76 Stockholm, Sweden ; Department of Clinical Sciences, Intervention and 
      Technology (CLINTEC), Section of Otorhinolaryngology, Karolinska Institutet, 
      Karolinska University Hospital, 171 76 Stockholm, Sweden ; Department of 
      Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical 
      University, Beijing 100730, China.
FAU - Palmgren, Bjorn
AU  - Palmgren B
AD  - Center for Hearing and Communication Research, Karolinska University Hospital, 
      171 76 Stockholm, Sweden ; Department of Clinical Sciences, Intervention and 
      Technology (CLINTEC), Section of Otorhinolaryngology, Karolinska Institutet, 
      Karolinska University Hospital, 171 76 Stockholm, Sweden.
FAU - Novozhilova, Ekaterina
AU  - Novozhilova E
AD  - Center for Hearing and Communication Research, Karolinska University Hospital, 
      171 76 Stockholm, Sweden ; Department of Clinical Sciences, Intervention and 
      Technology (CLINTEC), Section of Otorhinolaryngology, Karolinska Institutet, 
      Karolinska University Hospital, 171 76 Stockholm, Sweden.
FAU - Englund Johansson, Ulrica
AU  - Englund Johansson U
AD  - Department of Ophthalmology, Institution of Clinical Sciences in Lund, Lund 
      University, 221 84 Lund, Sweden.
FAU - Spieles-Engemann, Anne L
AU  - Spieles-Engemann AL
AD  - Center for Hearing and Communication Research, Karolinska University Hospital, 
      171 76 Stockholm, Sweden ; Department of Clinical Sciences, Intervention and 
      Technology (CLINTEC), Section of Otorhinolaryngology, Karolinska Institutet, 
      Karolinska University Hospital, 171 76 Stockholm, Sweden.
FAU - Kale, Ajay
AU  - Kale A
AD  - Center for Hearing and Communication Research, Karolinska University Hospital, 
      171 76 Stockholm, Sweden ; Department of Clinical Sciences, Intervention and 
      Technology (CLINTEC), Section of Otorhinolaryngology, Karolinska Institutet, 
      Karolinska University Hospital, 171 76 Stockholm, Sweden.
FAU - Stupp, Samuel I
AU  - Stupp SI
AD  - Departments of Chemistry, Materials Science and Engineering, and Medicine and 
      Institute for Bionanotechnology in Medicine, Northwestern University, Chicago, IL 
      60208, USA.
FAU - Olivius, Petri
AU  - Olivius P
AD  - Department of Clinical Sciences, Intervention and Technology (CLINTEC), Section 
      of Otorhinolaryngology, Karolinska Institutet, Karolinska University Hospital, 
      171 76 Stockholm, Sweden ; Division of Otorhinolaryngology, Linkoping University 
      Hospital, 581 85 Linkoping, Sweden ; Division of Otorhinolaryngology, Department 
      of Clinical and Experimental Medicine, University of Linkoping, 581 85 Linkoping, 
      Sweden.
LA  - eng
PT  - Journal Article
DEP - 20140826
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Gels)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Count
MH  - Cell Differentiation/*drug effects
MH  - Cell Survival/drug effects
MH  - Cochlear Nerve/drug effects/*pathology
MH  - Disease Models, Animal
MH  - Female
MH  - Gels/chemistry
MH  - Green Fluorescent Proteins/metabolism
MH  - Humans
MH  - Nanofibers/*chemistry
MH  - Neural Stem Cells/*cytology/drug effects/metabolism
MH  - Neurons/*cytology/drug effects/metabolism/*pathology
MH  - Rats, Sprague-Dawley
MH  - *Stem Cell Transplantation
PMC - PMC4160623
EDAT- 2014/09/23 06:00
MHDA- 2016/05/18 06:00
PMCR- 2014/08/26
CRDT- 2014/09/23 06:00
PHST- 2014/02/21 00:00 [received]
PHST- 2014/05/02 00:00 [revised]
PHST- 2014/07/29 00:00 [accepted]
PHST- 2014/09/23 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2016/05/18 06:00 [medline]
PHST- 2014/08/26 00:00 [pmc-release]
AID - 10.1155/2014/356415 [doi]
PST - ppublish
SO  - Biomed Res Int. 2014;2014:356415. doi: 10.1155/2014/356415. Epub 2014 Aug 26.