PMID- 25243407
OWN - NLM
STAT- MEDLINE
DCOM- 20151217
LR  - 20220321
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 9
DP  - 2014
TI  - Long non-coding RNA profiling in laryngeal squamous cell carcinoma and its 
      clinical significance: potential biomarkers for LSCC.
PG  - e108237
LID - 10.1371/journal.pone.0108237 [doi]
LID - e108237
AB  - Long non-coding RNAs (lncRNAs) are novel transcripts that may play important 
      roles in cancer. Our study aimed to resolve the lncRNA profile of larynx squamous 
      cell carcinoma (LSCC) and to determine its clinical significance. The global 
      lncRNA expression profile in LSCC tissues was measured by lncRNA microarray. 
      Distinctly expressed lncRNAs were identified and levels of AC026166.2-001 and 
      RP11-169D4.1-001 lncRNAs in 87 LSCC samples and paired adjacent normal tissue 
      were analyzed by real-time quantitative reverse transcriptase-polymerase chain 
      reaction (qRT-PCR). The clinical significance of these lncRNAs in laryngeal 
      cancer was analyzed and survival data were estimated by the Kaplan-Meier method 
      and the log-rank test. A receiver operating characteristic (ROC) curve was 
      constructed to check the diagnostic value. In the lncRNA expression profile of 
      tumor samples, 684 lncRNAs were upregulated and 747 lncRNAs were downregulated 
      (fold-change >2.0). Of these, AC026166.2-001 and RP11-169D4.1-001 were distinctly 
      dysregulated, with AC026166.2-001 exhibiting lower expression in cancer tissues 
      and RP11-169D4.1-001 higher expression. We verified that both AC026166.2-001 and 
      RP11-169D4.1-001 were expressed at a lower level in cervical lymph nodes compared 
      with paired laryngeal cancer tissues and paired normal tissues. RP11-169D4.1-001 
      levels were positively correlated with lymph node metastasis (P = 0.007). From 
      the survival analysis, decreased levels of AC026166.2-001 and RP11-169D4.1-001 
      were associated with poorer prognosis. The area under the ROC curve was up to 
      0.65 and 0.67, respectively, and the cut-off point of DeltaCt was 11.23 and 10.53, 
      respectively. AC026166.2-001 and RP11-169D4.1-001 may act as novel biomarkers in 
      LSCC and may be potential therapeutic targets for LSCC patients. Both 
      AC026166.2-001 and RP11-169D4.1-001 could be independent prognostic factors for 
      survival in LSCC.
FAU - Shen, Zhisen
AU  - Shen Z
AD  - Department of Otorhinolaryngology (Head and Neck Surgery), Lihuili Hospital of 
      Ningbo University, Ningbo, China.
FAU - Li, Qun
AU  - Li Q
AD  - Department of Otorhinolaryngology (Head and Neck Surgery), Lihuili Hospital of 
      Ningbo University, Ningbo, China; Ningbo University School of Medicine, Zhejiang 
      Provincial Key Laboratory of Pathophysiology, Ningbo, China.
FAU - Deng, Hongxia
AU  - Deng H
AD  - Department of Otorhinolaryngology (Head and Neck Surgery), Lihuili Hospital of 
      Ningbo University, Ningbo, China.
FAU - Lu, Dakai
AU  - Lu D
AD  - Ningbo University School of Medicine, Zhejiang Provincial Key Laboratory of 
      Pathophysiology, Ningbo, China.
FAU - Song, Haojun
AU  - Song H
AD  - Ningbo University School of Medicine, Zhejiang Provincial Key Laboratory of 
      Pathophysiology, Ningbo, China.
FAU - Guo, Junming
AU  - Guo J
AD  - Ningbo University School of Medicine, Zhejiang Provincial Key Laboratory of 
      Pathophysiology, Ningbo, China.
LA  - eng
SI  - GEO/GSE59652
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140922
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression Profiling
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Lymphatic Metastasis/genetics
MH  - Male
MH  - Middle Aged
MH  - Neck
MH  - RNA, Long Noncoding/*genetics
MH  - Survival Analysis
PMC - PMC4171522
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/09/23 06:00
MHDA- 2015/12/19 06:00
PMCR- 2014/09/22
CRDT- 2014/09/23 06:00
PHST- 2014/04/17 00:00 [received]
PHST- 2014/08/26 00:00 [accepted]
PHST- 2014/09/23 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2015/12/19 06:00 [medline]
PHST- 2014/09/22 00:00 [pmc-release]
AID - PONE-D-14-16940 [pii]
AID - 10.1371/journal.pone.0108237 [doi]
PST - epublish
SO  - PLoS One. 2014 Sep 22;9(9):e108237. doi: 10.1371/journal.pone.0108237. 
      eCollection 2014.