PMID- 25244359
OWN - NLM
STAT- MEDLINE
DCOM- 20160331
LR  - 20181202
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 90
IP  - 3
DP  - 2014 Dec
TI  - Identification and activity of acetate-assimilating bacteria in diffuse fluids 
      venting from two deep-sea hydrothermal systems.
PG  - 731-46
LID - 10.1111/1574-6941.12429 [doi]
AB  - Diffuse hydrothermal fluids often contain organic compounds such as hydrocarbons, 
      lipids, and organic acids. Microorganisms consuming these compounds at 
      hydrothermal sites are so far only known from cultivation-dependent studies. To 
      identify potential heterotrophs without prior cultivation, we combined microbial 
      community analysis with short-term incubations using (13)C-labeled acetate at two 
      distinct hydrothermal systems. We followed cell growth and assimilation of (13)C 
      into single cells by nanoSIMS combined with fluorescence in situ hybridization 
      (FISH). In 55  degrees C-fluids from the Menez Gwen hydrothermal system/Mid-Atlantic 
      Ridge, a novel epsilonproteobacterial group accounted for nearly all assimilation 
      of acetate, representing the first aerobic acetate-consuming member of the 
      Nautiliales. In contrast, Gammaproteobacteria dominated the (13) C-acetate 
      assimilation in incubations of 37  degrees C-fluids from the back-arc hydrothermal system 
      in the Manus Basin/Papua New Guinea. Here, 16S rRNA gene sequences were mostly 
      related to mesophilic Marinobacter, reflecting the high content of seawater in 
      these fluids. The rapid growth of microorganisms upon acetate addition suggests 
      that acetate consumers in diffuse fluids are copiotrophic opportunists, which 
      quickly exploit their energy sources, whenever available under the spatially and 
      temporally highly fluctuating conditions. Our data provide first insights into 
      the heterotrophic microbial community, catalyzing an under-investigated part of 
      microbial carbon cycling at hydrothermal vents.
CI  - (c) 2014 Federation of European Microbiological Societies. Published by John Wiley 
      & Sons Ltd. All rights reserved.
FAU - Winkel, Matthias
AU  - Winkel M
AD  - Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, 
      Bremen, Germany.
FAU - Pjevac, Petra
AU  - Pjevac P
FAU - Kleiner, Manuel
AU  - Kleiner M
FAU - Littmann, Sten
AU  - Littmann S
FAU - Meyerdierks, Anke
AU  - Meyerdierks A
FAU - Amann, Rudolf
AU  - Amann R
FAU - Mussmann, Marc
AU  - Mussmann M
LA  - eng
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PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141013
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Acetates)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Acetates/*metabolism
MH  - Aquatic Organisms/genetics
MH  - Base Sequence
MH  - Epsilonproteobacteria/classification/genetics/*metabolism
MH  - Gammaproteobacteria/classification/genetics/*metabolism
MH  - Heterotrophic Processes
MH  - Hydrothermal Vents/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Microbial Consortia/*genetics
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/microbiology
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - 16S rRNA gene
OT  - Epsilonproteobacteria
OT  - Gammaproteobacteria
OT  - heterotrophy
OT  - nanoSIMS
OT  - stable isotopes
EDAT- 2014/09/23 06:00
MHDA- 2016/04/01 06:00
CRDT- 2014/09/23 06:00
PHST- 2014/07/28 00:00 [received]
PHST- 2014/09/10 00:00 [revised]
PHST- 2014/09/16 00:00 [accepted]
PHST- 2014/09/23 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2016/04/01 06:00 [medline]
AID - 10.1111/1574-6941.12429 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2014 Dec;90(3):731-46. doi: 10.1111/1574-6941.12429. Epub 
      2014 Oct 13.