PMID- 25244497
OWN - NLM
STAT- MEDLINE
DCOM- 20160331
LR  - 20240109
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 90
IP  - 3
DP  - 2014 Dec
TI  - Soil type-dependent effects of a potential biocontrol inoculant on indigenous 
      bacterial communities in the rhizosphere of field-grown lettuce.
PG  - 718-30
LID - 10.1111/1574-6941.12430 [doi]
AB  - Bacterial biocontrol strains used as an alternative to chemical fungicides may 
      influence bacterial communities in the rhizosphere and effects might differ 
      depending on the soil type. Here we present baseline data on the effects of 
      Pseudomonas jessenii RU47 on the bacterial community composition in the 
      rhizosphere of lettuce grown in diluvial sand, alluvial loam and loess loam at 
      the same field site. 16S rRNA gene fragments amplified from total community DNA 
      were analyzed by denaturing gradient gel electrophoresis (DGGE) and 
      pyrosequencing. DGGE fingerprints revealed that in three consecutive years 
      (2010-2012) RU47 had a slight but statistically significant effect on the 
      bacterial community composition in one (2010), two (2011) or all the three soils 
      (2012). However, these effects were much less pronounced compared with the 
      influence of soil types. Additional pyrosequence analysis of samples from 2011 
      showed that significant changes in bacterial community compositions in response 
      to RU47 inoculation occurred only in alluvial loam. Different taxonomic groups 
      responded to the RU47 application depending on the soil type. Most remarkable was 
      the increased relative abundance of OTUs belonging to the genera Bacillus and 
      Paenibacillus in alluvial loam. Pyrosequencing allowed side-effects of the 
      application of bacterial inoculants into the rhizosphere to be identified.
CI  - (c) 2014 Federation of European Microbiological Societies. Published by John Wiley 
      & Sons Ltd. All rights reserved.
FAU - Schreiter, Susanne
AU  - Schreiter S
AD  - Julius Kuhn-Institut, Federal Research Centre for Cultivated Plants (JKI), 
      Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany; 
      Department Plant Health, Leibniz Institute of Vegetable and Ornamental Crops 
      Grossbeeren/Erfurt e.V., Grossbeeren, Germany.
FAU - Ding, Guo-Chun
AU  - Ding GC
FAU - Grosch, Rita
AU  - Grosch R
FAU - Kropf, Siegfried
AU  - Kropf S
FAU - Antweiler, Kai
AU  - Antweiler K
FAU - Smalla, Kornelia
AU  - Smalla K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141031
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil)
SB  - IM
MH  - Acidobacteria/classification/genetics
MH  - Actinobacteria/classification/genetics
MH  - Agricultural Inoculants/*classification/genetics
MH  - Bacteroidetes/classification/genetics
MH  - Base Sequence
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Lactuca/genetics/*microbiology
MH  - Microbial Consortia
MH  - Plant Roots/*microbiology
MH  - Proteobacteria/classification/genetics
MH  - Pseudomonas/*physiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rhizosphere
MH  - Sequence Analysis, DNA
MH  - Soil/classification
MH  - *Soil Microbiology
OTO - NOTNLM
OT  - 16S rRNA gene
OT  - Pseudomonas jesseniiRU47
OT  - biocontrol
OT  - denaturing gradient gel electrophoresis
OT  - pyrosequencing
OT  - total community DNA
EDAT- 2014/09/23 06:00
MHDA- 2016/04/01 06:00
CRDT- 2014/09/23 06:00
PHST- 2014/07/09 00:00 [received]
PHST- 2014/09/09 00:00 [revised]
PHST- 2014/09/14 00:00 [accepted]
PHST- 2014/09/23 06:00 [entrez]
PHST- 2014/09/23 06:00 [pubmed]
PHST- 2016/04/01 06:00 [medline]
AID - 10.1111/1574-6941.12430 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2014 Dec;90(3):718-30. doi: 10.1111/1574-6941.12430. Epub 
      2014 Oct 31.