PMID- 25246909
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140923
LR  - 20211021
IS  - 1680-6433 (Print)
IS  - 2008-2177 (Electronic)
IS  - 1680-6433 (Linking)
VI  - 10
IP  - 5
DP  - 2012 Sep
TI  - Effect of combined density gradient centrifugation on X- and Y- sperm separation 
      and chromatin integrity.
PG  - 435-40
AB  - BACKGROUND: It has been claimed that by using different washing methods, the 
      sperms can be separated according to size, motility, density, chromosomal content 
      and surface markings and charge. These methods also reduce sperm chromatin 
      deficiencies and screen the sperms before applying in assisted reproduction 
      techniques. OBJECTIVE: This study compared simple density gradient methods and a 
      combined method with albumin density gradient and PureSperm separation 
      (alb/PureSperm) for sex preselection by double fluorescence in situ hybridization 
      (FISH) versus chromomycin A3 staining to determine chromatin integrity. MATERIALS 
      AND METHODS: 30 normal semen samples were prepared with PureSperm, albumin 
      gradients and alb/PureSperm. All samples were then stained by FISH and 
      chromomycin A3. The results were compared with SPSS 11.5 and the Kruskal-Wallis 
      test. RESULTS: The proportion of X-bearing spermatozoa by PureSperm separation 
      (47.58+/-5.67) and Y-bearing spermatozoa by albumin gradient (46.13+/-3.83) methods 
      were slightly higher than in putative normal sperm samples (1:1), but there were 
      no significant differences in the X- or Y- bearing spermatozoa counts among the 
      three methods. Albumin gradient separation tended to underestimate abnormal 
      spermatozoa compared to PureSperm and combined alb/PureSperm. CONCLUSION: Routine 
      separation methods slightly enriched X- or Y- bearing spermatozoa, but the 
      differences were not significant for clinical purposes. The combined 
      alb/PureSperm method had no advantages for assessing sex ratio or chromatin 
      integrity compared to simpler gradient methods.
FAU - Esmaeilpour, Tahereh
AU  - Esmaeilpour T
AD  - Department of Anatomical Sciences, Medical School, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Elyasi, Leila
AU  - Elyasi L
AD  - Department of Anatomical Sciences, Medical School, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Bahmanpour, Soghra
AU  - Bahmanpour S
AD  - Department of Anatomical Sciences, Medical School, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Ghannadi, Alireza
AU  - Ghannadi A
AD  - Islamic Azad University, Larestan, Iran. ; Shiraz Infertility Center, Shiraz, 
      Iran.
FAU - Monabbati, Ahmad
AU  - Monabbati A
AD  - Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran.
FAU - Dehghani, Farzaneh
AU  - Dehghani F
AD  - Department of Anatomical Sciences, Medical School, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Kazerooni, Marjaneh
AU  - Kazerooni M
AD  - Shiraz Infertility Center, Shiraz, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Reprod Med
JT  - Iranian journal of reproductive medicine
JID - 101463749
PMC - PMC4169681
OTO - NOTNLM
OT  - Albumin gradient
OT  - Double-labeled fluorescence in situ hybridization
OT  - Sex ratio
OT  - Sperm chromatin
OT  - Sperm separation
EDAT- 2012/09/01 00:00
MHDA- 2012/09/01 00:01
PMCR- 2012/09/01
CRDT- 2014/09/24 06:00
PHST- 2011/07/31 00:00 [received]
PHST- 2011/10/08 00:00 [revised]
PHST- 2011/12/14 00:00 [accepted]
PHST- 2014/09/24 06:00 [entrez]
PHST- 2012/09/01 00:00 [pubmed]
PHST- 2012/09/01 00:01 [medline]
PHST- 2012/09/01 00:00 [pmc-release]
AID - ijrm-10-435 [pii]
PST - ppublish
SO  - Iran J Reprod Med. 2012 Sep;10(5):435-40.