PMID- 25247469
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20141122
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Linking)
VI  - 155
IP  - 12
DP  - 2014 Dec
TI  - Kiss1 neurons drastically change their firing activity in accordance with the 
      reproductive state: insights from a seasonal breeder.
PG  - 4868-80
LID - 10.1210/en.2014-1472 [doi]
AB  - Kisspeptin (Kiss) neurons show drastic changes in kisspeptin expression in 
      response to the serum sex steroid concentration in various vertebrate species. 
      Thus, according to the reproductive states, kisspeptin neurons are suggested to 
      modulate various neuronal activities, including the regulation of GnRH neurons in 
      mammals. However, despite their reproductive state-dependent regulation, there is 
      no physiological analysis of kisspeptin neurons in seasonal breeders. Here we 
      generated the first kiss1-enhanced green fluorescent protein transgenic line of a 
      seasonal breeder, medaka, for histological and electrophysiological analyses 
      using a whole-brain in vitro preparation in which most synaptic connections are 
      intact. We found histologically that Kiss1 neurons in the nucleus ventralis 
      tuberis (NVT) projected to the preoptic area, hypothalamus, pituitary, and 
      ventral telencephalon. Therefore, NVT Kiss1 neurons may regulate various 
      homeostatic functions and innate behaviors. Electrophysiological analyses 
      revealed that they show various firing patterns, including bursting. Furthermore, 
      we found that their firings are regulated by the resting membrane potential. 
      However, bursting was not induced from the other firing patterns with a current 
      injection, suggesting that it requires some chronic modulations of intrinsic 
      properties such as channel expression. Finally, we found that NVT Kiss1 neurons 
      drastically change their neuronal activities according to the reproductive state 
      and the estradiol levels. Taken together with the previous reports, we here 
      conclude that the breeding condition drastically alters the Kiss1 neuron 
      activities in both gene expression and firing activities, the latter of which is 
      strongly related to Kiss1 release, and the Kiss1 peptides regulate the activities 
      of various neural circuits through their axonal projections.
FAU - Hasebe, Masaharu
AU  - Hasebe M
AD  - Department of Biological Sciences (M.H., S.K., H.S., Y.A., Y.O.), Graduate School 
      of Science, The University of Tokyo, Tokyo 113-0033, Japan; and Laboratory of 
      Fish Biology (H.A.), Graduate School of Bioagricultural Sciences, Nagoya 
      University, Nagoya 464-8601, Japan.
FAU - Kanda, Shinji
AU  - Kanda S
FAU - Shimada, Hiroyuki
AU  - Shimada H
FAU - Akazome, Yasuhisa
AU  - Akazome Y
FAU - Abe, Hideki
AU  - Abe H
FAU - Oka, Yoshitaka
AU  - Oka Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140923
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Estrogens)
RN  - 0 (Kisspeptins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Estradiol/physiology
MH  - Estrogens
MH  - Female
MH  - Green Fluorescent Proteins
MH  - Kisspeptins/*physiology
MH  - Male
MH  - Membrane Potentials
MH  - Neurons/*physiology
MH  - Oryzias
MH  - Patch-Clamp Techniques
MH  - *Reproduction
EDAT- 2014/09/24 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/09/24 06:00
PHST- 2014/09/24 06:00 [entrez]
PHST- 2014/09/24 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.1210/en.2014-1472 [doi]
PST - ppublish
SO  - Endocrinology. 2014 Dec;155(12):4868-80. doi: 10.1210/en.2014-1472. Epub 2014 Sep 
      23.