PMID- 25248041
OWN - NLM
STAT- MEDLINE
DCOM- 20150511
LR  - 20141230
IS  - 1538-943X (Electronic)
IS  - 1058-2916 (Linking)
VI  - 61
IP  - 1
DP  - 2015 Jan-Feb
TI  - Improved in vitro biocompatibility of surface-modified hydroxyapatite sponge 
      scaffold with gelatin and BMP-2 in comparison against a commercial bone 
      allograft.
PG  - 78-86
LID - 10.1097/MAT.0000000000000155 [doi]
AB  - This study aims to demonstrate the morphology and in vitro biocompatibility of 
      neat and surface-modified hydroxyapatite sponge scaffold (SM-HASS) which was 
      fabricated using a sponge replica method, and compared with the commercially 
      available demineralized freeze-dried bone allograft (DFDBA). 
      Surface-modifications were done by coating the surface area of the neat 
      hydroxyapatite sponge scaffold (HASS) with either gelatin alone (HASS/G) or 
      gelatin and BMP-2 growth factor (HASS/G+B). Scanning electron microscope (SEM), 
      Fourier transform infrared (FTIR), porosity, pore size distribution, and 
      compressive strength analyses showed that the addition of gelatin in HASS/G 
      produced a morphologically and structurally similar scaffold to that of the 
      allograft. The addition of BMP-2 improved the biocompatibility of the HASS/G+B in 
      vitro using MC3T3-E1 cells which showed better cell viability, proliferation, and 
      cell adhesion than on the allograft. Therefore, hydroxyapatite scaffold coated 
      with gelatin polymer and gelatin with BMP-2 growth factor showed comparable 
      performance against commercially available DFDBA from cadaver with regards to 
      structure and in vitro biocompatibility.
FAU - Carpena, Nathaniel T
AU  - Carpena NT
AD  - From the *InoBone, Asan, Chungcheongnam-do, South Korea; and daggerDepartment of 
      Regenerative Medicine, double daggerDepartment of Physiology, and  section signInstitute of Tissue 
      Regeneration, College of Medicine, SoonChunHyang University, Cheonan, South 
      Korea.
FAU - Min, Young-Ki
AU  - Min YK
FAU - Lee, Byong-Taek
AU  - Lee BT
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - ASAIO J
JT  - ASAIO journal (American Society for Artificial Internal Organs : 1992)
JID - 9204109
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Substitutes)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Recombinant Proteins)
RN  - 9000-70-8 (Gelatin)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - 3T3 Cells
MH  - Allografts
MH  - Animals
MH  - Bone Morphogenetic Protein 2/*administration & dosage
MH  - *Bone Substitutes/chemistry
MH  - *Bone Transplantation
MH  - Cell Adhesion
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Coated Materials, Biocompatible/chemistry
MH  - Compressive Strength
MH  - Durapatite/chemistry
MH  - Gelatin/chemistry
MH  - Humans
MH  - In Vitro Techniques
MH  - Materials Testing
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Osteoblasts/cytology
MH  - Porosity
MH  - Recombinant Proteins/administration & dosage
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Tissue Scaffolds/*chemistry
EDAT- 2014/09/24 06:00
MHDA- 2015/05/12 06:00
CRDT- 2014/09/24 06:00
PHST- 2014/09/24 06:00 [entrez]
PHST- 2014/09/24 06:00 [pubmed]
PHST- 2015/05/12 06:00 [medline]
AID - 10.1097/MAT.0000000000000155 [doi]
PST - ppublish
SO  - ASAIO J. 2015 Jan-Feb;61(1):78-86. doi: 10.1097/MAT.0000000000000155.