PMID- 25248636 OWN - NLM STAT- MEDLINE DCOM- 20150406 LR - 20211021 IS - 1617-4623 (Electronic) IS - 1617-4623 (Linking) VI - 290 IP - 1 DP - 2015 Feb TI - Diversity within the genus Elymus (Poaceae: Triticeae) as investigated by the analysis of the nr5S rDNA variation in species with St and H haplomes. PG - 329-42 LID - 10.1007/s00438-014-0907-4 [doi] AB - The genus Elymus ("Ryegrass") is a repository for a range of species with a variety of haplome contents; hence the pejorative name "dustbin" genus. We have analyzed 1,059 sequences from 128 accessions representing 24 species to investigate the relationships among the StH haplomes-containing species described by Yen and Yang (Genus Elymus Beijing 5:58-362, 2013). Sequences were assigned to "unit classes" of orthologous sequences and subjected to a suite of analyses including BLAST (Basic Local Alignment Search Tool) searches, phylogenetic analysis and population genetic analysis to estimate species diversity. Our results support the genome analyses in Yen and Yang (Genus Elymus Beijing 5:58-362, 2013), i.e., genomic constitution StStHH including variants restricted to Elymus. Population genetic analysis of the 5S nrDNA sequence data revealed that the within-species variance component is roughly +/-89 %; thus, we were unable to identify molecular markers capable to separate the 24 species analyzed. Separate phylogenetic analyses of the two unit classes and of all the data exhibit a trend only of the species to cluster on the phylograms. Finally, the analysis provides evidence for the multiple origins of American and Eurasian species. FAU - Baum, B R AU - Baum BR AD - Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, ON, K1A OC6, Canada, bernard.baum@agr.gc.ca. FAU - Edwards, T AU - Edwards T FAU - Johnson, D A AU - Johnson DA LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140924 PL - Germany TA - Mol Genet Genomics JT - Molecular genetics and genomics : MGG JID - 101093320 RN - 0 (DNA, Ribosomal) SB - IM MH - Base Sequence MH - Bayes Theorem MH - DNA, Ribosomal/*genetics MH - Elymus/*genetics MH - *Genetic Variation MH - Genetics, Population MH - Haplotypes/*genetics MH - Models, Genetic MH - Phylogeny MH - *Polyploidy MH - Principal Component Analysis MH - Species Specificity EDAT- 2014/09/25 06:00 MHDA- 2015/04/07 06:00 CRDT- 2014/09/25 06:00 PHST- 2014/05/27 00:00 [received] PHST- 2014/08/23 00:00 [accepted] PHST- 2014/09/25 06:00 [entrez] PHST- 2014/09/25 06:00 [pubmed] PHST- 2015/04/07 06:00 [medline] AID - 10.1007/s00438-014-0907-4 [doi] PST - ppublish SO - Mol Genet Genomics. 2015 Feb;290(1):329-42. doi: 10.1007/s00438-014-0907-4. Epub 2014 Sep 24.