PMID- 25248645
OWN - NLM
STAT- MEDLINE
DCOM- 20160515
LR  - 20211021
IS  - 2040-3372 (Electronic)
IS  - 2040-3364 (Print)
IS  - 2040-3364 (Linking)
VI  - 6
IP  - 21
DP  - 2014 Nov 7
TI  - Dual-modality, fluorescent, PLGA encapsulated bismuth nanoparticles for molecular 
      and cellular fluorescence imaging and computed tomography.
PG  - 13104-12
LID - 10.1039/c4nr01405g [doi]
AB  - Reports of molecular and cellular imaging using computed tomography (CT) are 
      rapidly increasing. Many of these reports use gold nanoparticles. Bismuth has 
      similar CT contrast properties to gold while being approximately 1000-fold less 
      expensive. Herein we report the design, fabrication, characterization, and CT and 
      fluorescence imaging properties of a novel, dual modality, fluorescent, polymer 
      encapsulated bismuth nanoparticle construct for computed tomography and 
      fluorescence imaging. We also report on cellular internalization and preliminary 
      in vitro and in vivo toxicity effects of these constructs. 40 nm bismuth(0) 
      nanocrystals were synthesized and encapsulated within 120 nm 
      Poly(dl-lactic-co-glycolic acid) (PLGA) nanoparticles by oil-in-water emulsion 
      methodologies. Coumarin-6 was co-encapsulated to impart fluorescence. High 
      encapsulation efficiency was achieved  approximately 70% bismuth w/w. Particles were shown to 
      internalize within cells following incubation in culture. Bismuth nanocrystals 
      and PLGA encapsulated bismuth nanoparticles exhibited >90% and >70% degradation, 
      respectively, within 24 hours in acidic, lysosomal environment mimicking media 
      and both remained nearly 100% stable in cytosolic/extracellular fluid mimicking 
      media. muCT and clinical CT imaging was performed at multiple X-ray tube voltages 
      to measure concentration dependent attenuation rates as well as to establish the 
      ability to detect the nanoparticles in an ex vivo biological sample. Dual 
      fluorescence and CT imaging is demonstrated as well. In vivo toxicity studies in 
      rats revealed neither clinically apparent side effects nor major alterations in 
      serum chemistry and hematology parameters. Calculations on minimal detection 
      requirements for in vivo targeted imaging using these nanoparticles are 
      presented. Indeed, our results indicate that these nanoparticles may serve as a 
      platform for sensitive and specific targeted molecular CT and fluorescence 
      imaging.
FAU - Swy, Eric R
AU  - Swy ER
AD  - Department of Radiology, Michigan State University, East Lansing, MI 48824, USA. 
      erik.shapiro@rad.msu.edu.
FAU - Schwartz-Duval, Aaron S
AU  - Schwartz-Duval AS
FAU - Shuboni, Dorela D
AU  - Shuboni DD
FAU - Latourette, Matthew T
AU  - Latourette MT
FAU - Mallet, Christiane L
AU  - Mallet CL
FAU - Parys, Maciej
AU  - Parys M
FAU - Cormode, David P
AU  - Cormode DP
FAU - Shapiro, Erik M
AU  - Shapiro EM
LA  - eng
GR  - DP2 OD004362/OD/NIH HHS/United States
GR  - R00 EB012165/EB/NIBIB NIH HHS/United States
GR  - DP2 OD001886/OD/NIH HHS/United States
GR  - R21 EB017881/EB/NIBIB NIH HHS/United States
GR  - DP2 OD001880/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Nanoscale
JT  - Nanoscale
JID - 101525249
RN  - 0 (Contrast Media)
RN  - 0 (Coumarins)
RN  - 0 (Thiazoles)
RN  - 0 (coumarin 6)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - U015TT5I8H (Bismuth)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Bismuth/*chemistry
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Chickens
MH  - Contrast Media/chemistry
MH  - Coumarins/chemistry
MH  - Lactic Acid/*chemistry
MH  - Lysosomes/metabolism
MH  - Male
MH  - Metal Nanoparticles/*chemistry
MH  - Mice
MH  - Microscopy, Electron, Transmission
MH  - *Microscopy, Fluorescence
MH  - Nanoparticles/chemistry
MH  - Nanotechnology
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Spectrometry, Fluorescence
MH  - Temperature
MH  - Thiazoles/chemistry
MH  - *Tomography, X-Ray Computed
MH  - X-Ray Microtomography
PMC - PMC4362618
MID - NIHMS630841
EDAT- 2014/09/25 06:00
MHDA- 2016/05/18 06:00
PMCR- 2015/11/07
CRDT- 2014/09/25 06:00
PHST- 2014/09/25 06:00 [entrez]
PHST- 2014/09/25 06:00 [pubmed]
PHST- 2016/05/18 06:00 [medline]
PHST- 2015/11/07 00:00 [pmc-release]
AID - 10.1039/c4nr01405g [doi]
PST - ppublish
SO  - Nanoscale. 2014 Nov 7;6(21):13104-12. doi: 10.1039/c4nr01405g.