PMID- 25249772
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140924
LR  - 20211021
IS  - 1415-4757 (Print)
IS  - 1678-4685 (Electronic)
IS  - 1415-4757 (Linking)
VI  - 37
IP  - 3
DP  - 2014 Sep
TI  - Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian 
      carp.
PG  - 500-7
AB  - Reference genes are commonly used for normalization of target gene expression 
      during RT-qPCR analysis. However, no housekeeping genes or reference genes have 
      been identified to be stable across different tissue types or under different 
      experimental conditions. To identify the most suitable reference genes for 
      RT-qPCR analysis of target gene expression in the hepatopancreas of crucian carp 
      (Carassius auratus) under various conditions (sex, age, water temperature, and 
      drug treatments), seven reference genes, including beta actin (ACTB), beta-2 
      microglobulin (B2M), embryonic elongation factor-1 alpha (EEF1A), glyceraldehyde 
      phosphate dehydrogenase (GAPDH), alpha tubulin (TUBA), ribosomal protein l8 
      (RPL8) and glucose-6-phosphate dehydrogenase (G6PDH), were evaluated in this 
      study. The stability and ranking of gene expression were analyzed using three 
      different statistical programs: GeNorm, Normfinder and Bestkeeper. The expression 
      errors associated with selection of the genes were assessed by the relative 
      quantity of CYP4T. The results indicated that all the seven genes exhibited 
      variability under the experimental conditions of this research, and the 
      combination of ACTB/TUBA/EEF1A or of ACTB/EEF1A was the best candidate that 
      raised the accuracy of quantitative analysis of gene expression. The findings 
      highlighted the importance of validation of housekeeping genes for research on 
      gene expression under different conditions of experiment and species.
FAU - Mo, Fei
AU  - Mo F
AD  - Laboratory of Veterinary Pharmacology and Toxicology , College of Veterinary 
      Medicine , Nanjing Agricultural University , Nanjing, Jiangsu Province , China .
FAU - Zhao, Jie
AU  - Zhao J
AD  - Laboratory of Veterinary Pharmacology and Toxicology , College of Veterinary 
      Medicine , Nanjing Agricultural University , Nanjing, Jiangsu Province , China .
FAU - Liu, Na
AU  - Liu N
AD  - Laboratory of Veterinary Pharmacology and Toxicology , College of Veterinary 
      Medicine , Nanjing Agricultural University , Nanjing, Jiangsu Province , China .
FAU - Cao, Li-Hua
AU  - Cao LH
AD  - Laboratory of Veterinary Pharmacology and Toxicology , College of Veterinary 
      Medicine , Nanjing Agricultural University , Nanjing, Jiangsu Province , China . 
      ; Guangdong Agribusiness Group Corporation , Guangzhou, Guangdong Province , 
      China .
FAU - Jiang, Shan-Xiang
AU  - Jiang SX
AD  - Laboratory of Veterinary Pharmacology and Toxicology , College of Veterinary 
      Medicine , Nanjing Agricultural University , Nanjing, Jiangsu Province , China .
LA  - eng
PT  - Journal Article
PL  - Brazil
TA  - Genet Mol Biol
JT  - Genetics and molecular biology
JID - 100883590
PMC - PMC4171773
OTO - NOTNLM
OT  - CYP4T
OT  - crucian carp
OT  - gene expression
OT  - housekeeping genes
OT  - quantitative RT-PCR
EDAT- 2014/09/25 06:00
MHDA- 2014/09/25 06:01
PMCR- 2014/09/01
CRDT- 2014/09/25 06:00
PHST- 2013/09/18 00:00 [received]
PHST- 2014/01/24 00:00 [accepted]
PHST- 2014/09/25 06:00 [entrez]
PHST- 2014/09/25 06:00 [pubmed]
PHST- 2014/09/25 06:01 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - gmb-37-500 [pii]
AID - 10.1590/s1415-47572014000400005 [doi]
PST - ppublish
SO  - Genet Mol Biol. 2014 Sep;37(3):500-7. doi: 10.1590/s1415-47572014000400005.