PMID- 25250214
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140924
LR  - 20211021
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 2
DP  - 2014
TI  - Identification of a truncated splice variant of IL-18 receptor alpha in the human 
      and rat, with evidence of wider evolutionary conservation.
PG  - e560
LID - 10.7717/peerj.560 [doi]
LID - e560
AB  - Interleukin-18 (IL-18) is a pro-inflammatory cytokine which stimulates activation 
      of the nuclear factor kappa beta (NF-kappaB) pathway via interaction with the IL-18 
      receptor. The receptor itself is formed from a dimer of two subunits, with the 
      ligand-binding IL-18Ralpha subunit being encoded by the IL18R1 gene. A splice variant 
      of murine IL18r1, which has been previously described, is formed by transcription 
      of an unspliced intron (forming a 'type II' IL18r1 transcript) and is predicted 
      to encode a receptor with a truncated intracellular domain lacking the capacity 
      to generate downstream signalling. In order to examine the relevance of this 
      finding to human IL-18 function, we assessed the presence of a homologous 
      transcript by reverse transcription-polymerase chain reaction (RT-PCR) in the 
      human and rat as another common laboratory animal. We present evidence for type 
      II IL18R1 transcripts in both species. While the mouse and rat transcripts are 
      predicted to encode a truncated receptor with a novel 5 amino acid C-terminal 
      domain, the human sequence is predicted to encode a truncated protein with a 
      novel 22 amino acid sequence bearing resemblance to the 'Box 1' motif of the 
      Toll/interleukin-1 receptor (TIR) domain, in a similar fashion to the inhibitory 
      interleukin-1 receptor 2. Given that transcripts from these three species are all 
      formed by inclusion of homologous unspliced intronic regions, an analysis of 
      homologous introns across a wider array of 33 species with available IL18R1 gene 
      records was performed, which suggests similar transcripts may encode truncated 
      type II IL-18Ralpha subunits in other species. This splice variant may represent a 
      conserved evolutionary mechanism for regulating IL-18 activity.
FAU - Booker, Chris S
AU  - Booker CS
AD  - Centre for Neuroendocrinology, Department of Anatomy, University of Otago , 
      Dunedin , New Zealand.
FAU - Grattan, David R
AU  - Grattan DR
AD  - Centre for Neuroendocrinology, Department of Anatomy, University of Otago , 
      Dunedin , New Zealand.
LA  - eng
PT  - Journal Article
DEP - 20140911
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC4168765
OTO - NOTNLM
OT  - Comparative genomics
OT  - Interleukin-18
OT  - Splice variant
OT  - Toll-like receptor
EDAT- 2014/09/25 06:00
MHDA- 2014/09/25 06:01
PMCR- 2014/09/11
CRDT- 2014/09/25 06:00
PHST- 2014/05/31 00:00 [received]
PHST- 2014/08/15 00:00 [accepted]
PHST- 2014/09/25 06:00 [entrez]
PHST- 2014/09/25 06:00 [pubmed]
PHST- 2014/09/25 06:01 [medline]
PHST- 2014/09/11 00:00 [pmc-release]
AID - 560 [pii]
AID - 10.7717/peerj.560 [doi]
PST - epublish
SO  - PeerJ. 2014 Sep 11;2:e560. doi: 10.7717/peerj.560. eCollection 2014.