PMID- 25250485
OWN - NLM
STAT- MEDLINE
DCOM- 20150714
LR  - 20141201
IS  - 1531-4332 (Electronic)
IS  - 1095-6433 (Linking)
VI  - 179
DP  - 2015 Jan
TI  - mRNA levels of kisspeptins, kisspeptin receptors, and GnRH1 in the brain of chub 
      mackerel during puberty.
PG  - 104-12
LID - S1095-6433(14)00198-6 [pii]
LID - 10.1016/j.cbpa.2014.09.012 [doi]
AB  - Kisspeptin (Kiss) and its cognate receptor (Kiss1R), implicated in the 
      neuroendocrine control of GnRH secretion in mammals, have been proposed to be the 
      key factors in regulating puberty. However, the mechanisms underlying the 
      initiation of puberty in fish are poorly understood. The chub mackerel Scomber 
      japonicus expresses two forms of Kiss (kiss1 and kiss2) and two Kiss receptor 
      (kissr1 and kissr2) genes in the brain, which exhibit sexually dimorphic changes 
      during the seasonal reproductive cycle. This indicates that the kisspeptin system 
      plays an important role in gonadal recrudescence of chub mackerel; however, the 
      involvement of the kisspeptin system in the pubertal process has not been 
      identified. In the present study, we examined the mRNA expression of kiss1, 
      kiss2, kissr1, kissr2, and gnrh1 (hypophysiotropic form) in the brain of a chub 
      mackerel during puberty. In male fish, kiss2, kissr1 and kissr2 levels increased 
      significantly at 14weeks post-hatch (wph), synchronously with an increase in type 
      A spermatogonial populations in the testis; kiss2 and gnrh1 levels significantly 
      increased at 22wph, just before the onset of meiosis in the testes. In female 
      fish, kiss2 increased significantly at 14wph, synchronously with an increase in 
      the number of perinucleolar oocytes in the ovary; kiss1 and kiss2 levels 
      significantly increased concomitantly with an increase in the kissr1, kissr2, and 
      gnrh1 levels at 24wph, just before the onset of vitellogenesis in oocytes. The 
      present results suggest positive involvement of the kisspeptin-GnRH system in the 
      pubertal process in the captive reared chub mackerel.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Ohga, Hirofumi
AU  - Ohga H
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      812-8581, Japan.
FAU - Adachi, Hayato
AU  - Adachi H
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      812-8581, Japan.
FAU - Matsumori, Kojiro
AU  - Matsumori K
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      812-8581, Japan.
FAU - Kodama, Ryoko
AU  - Kodama R
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      812-8581, Japan.
FAU - Nyuji, Mitsuo
AU  - Nyuji M
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      812-8581, Japan.
FAU - Selvaraj, Sethu
AU  - Selvaraj S
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      812-8581, Japan.
FAU - Kato, Keitaro
AU  - Kato K
AD  - Fisheries Laboratory of Kinki University, Shirahama, Wakayama 649-2211, Japan.
FAU - Yamamoto, Shinji
AU  - Yamamoto S
AD  - Fisheries Laboratory of Kinki University, Shirahama, Wakayama 649-2211, Japan.
FAU - Yamaguchi, Akihiko
AU  - Yamaguchi A
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      812-8581, Japan.
FAU - Matsuyama, Michiya
AU  - Matsuyama M
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      812-8581, Japan. Electronic address: rinya_m@agr.kyushu-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140922
PL  - United States
TA  - Comp Biochem Physiol A Mol Integr Physiol
JT  - Comparative biochemistry and physiology. Part A, Molecular & integrative 
      physiology
JID - 9806096
RN  - 0 (Kisspeptins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Gonadotropin-Releasing Hormone/*genetics/metabolism
MH  - Gonads/growth & development/metabolism
MH  - Kisspeptins/*genetics/metabolism
MH  - Male
MH  - Perciformes/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, G-Protein-Coupled/*genetics/metabolism
MH  - Sexual Maturation/*genetics
OTO - NOTNLM
OT  - GnRH1
OT  - Kisspeptin
OT  - Kisspeptin receptor
OT  - Puberty
OT  - Scombridae
OT  - Teleost
EDAT- 2014/09/25 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/09/25 06:00
PHST- 2014/04/16 00:00 [received]
PHST- 2014/08/09 00:00 [revised]
PHST- 2014/09/15 00:00 [accepted]
PHST- 2014/09/25 06:00 [entrez]
PHST- 2014/09/25 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - S1095-6433(14)00198-6 [pii]
AID - 10.1016/j.cbpa.2014.09.012 [doi]
PST - ppublish
SO  - Comp Biochem Physiol A Mol Integr Physiol. 2015 Jan;179:104-12. doi: 
      10.1016/j.cbpa.2014.09.012. Epub 2014 Sep 22.