PMID- 25250714
OWN - NLM
STAT- MEDLINE
DCOM- 20151215
LR  - 20220309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 9
DP  - 2014
TI  - Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo.
PG  - e108697
LID - 10.1371/journal.pone.0108697 [doi]
LID - e108697
AB  - Genetically-encoded calcium indicators (GECIs) facilitate imaging activity of 
      genetically defined neuronal populations in vivo. The high intracellular GECI 
      concentrations required for in vivo imaging are usually achieved by viral gene 
      transfer using adeno-associated viruses. Transgenic expression of GECIs promises 
      important advantages, including homogeneous, repeatable, and stable expression 
      without the need for invasive virus injections. Here we present the generation 
      and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f 
      under the Thy1 promoter. We quantified GCaMP6 expression across brain regions and 
      neurons and compared to other transgenic mice and AAV-mediated expression. We 
      tested three mouse lines for imaging in the visual cortex in vivo and compared 
      their performance to mice injected with AAV expressing GCaMP6. Furthermore, we 
      show that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity 
      imaging in behaving mice.
FAU - Dana, Hod
AU  - Dana H
AD  - Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 
      United States of America.
FAU - Chen, Tsai-Wen
AU  - Chen TW
AD  - Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 
      United States of America.
FAU - Hu, Amy
AU  - Hu A
AD  - Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 
      United States of America.
FAU - Shields, Brenda C
AU  - Shields BC
AD  - Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 
      United States of America.
FAU - Guo, Caiying
AU  - Guo C
AD  - Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 
      United States of America.
FAU - Looger, Loren L
AU  - Looger LL
AD  - Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 
      United States of America.
FAU - Kim, Douglas S
AU  - Kim DS
AD  - Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 
      United States of America.
FAU - Svoboda, Karel
AU  - Svoboda K
AD  - Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 
      United States of America.
LA  - eng
GR  - Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140924
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - Calcium/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Neurons/*cytology/metabolism
MH  - Visual Cortex/cytology/physiology
PMC - PMC4177405
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/09/25 06:00
MHDA- 2015/12/17 06:00
PMCR- 2014/09/24
CRDT- 2014/09/25 06:00
PHST- 2014/07/13 00:00 [received]
PHST- 2014/08/11 00:00 [accepted]
PHST- 2014/09/25 06:00 [entrez]
PHST- 2014/09/25 06:00 [pubmed]
PHST- 2015/12/17 06:00 [medline]
PHST- 2014/09/24 00:00 [pmc-release]
AID - PONE-D-14-31023 [pii]
AID - 10.1371/journal.pone.0108697 [doi]
PST - epublish
SO  - PLoS One. 2014 Sep 24;9(9):e108697. doi: 10.1371/journal.pone.0108697. 
      eCollection 2014.