PMID- 25251136
OWN - NLM
STAT- MEDLINE
DCOM- 20160602
LR  - 20150602
IS  - 1097-0010 (Electronic)
IS  - 0022-5142 (Linking)
VI  - 95
IP  - 10
DP  - 2015 Aug 15
TI  - Rutin upregulates neurotrophic factors resulting in attenuation of 
      ethanol-induced oxidative stress in HT22 hippocampal neuronal cells.
PG  - 2117-23
LID - 10.1002/jsfa.6927 [doi]
AB  - BACKGROUND: Alcoholism, which refers to the excessive consumption of alcohol, has 
      deleterious effects on personal and social health worldwide. Oxidative stress 
      evoked by ethanol plays an important role in the pathogenesis of 
      neurodegenerative diseases. Rutin is a bioflavonoid that has been demonstrated to 
      scavenge superoxide radicals. However, the effects of rutin on neuronal toxicity 
      following ethanol-induced oxidative stress have not previously been investigated. 
      Thus we investigated the antioxidant effect of rutin in hippocampal neuronal 
      cells (HT22 cells) exposed to ethanol. RESULTS: We found that rutin pretreatment 
      prevented the ethanol-induced decrease in protein level expression of nerve 
      growth factor, glial cell line-derived neurotrophic factor (GDNF) and 
      brain-derived neurotrophic factor (BDNF) in HT22 cells. Cell viability as 
      analyzed by the MTT method revealed a significant increase in cell viability in 
      the rutin-treated group compared with the ethanol-only treated group. Antioxidant 
      effect of rutin was confirmed to be due to reduction of intracellular reactive 
      oxidative species production in ethanol-treated HT22 cells. Moreover, rutin 
      significantly increased the level of the antioxidant glutathione, and the 
      activities of the antioxidant enzymes superoxide dismutase and catalase. 
      CONCLUSION: These findings indicate that rutin has potential as a therapeutic 
      agent to treat alcohol-related neurodegenerative disorders.
CI  - (c) 2014 Society of Chemical Industry.
FAU - Song, Kibbeum
AU  - Song K
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk 
      National University, Jeonju, Jeonbuk 561-156, Republic of Korea.
FAU - Na, Ji-Young
AU  - Na JY
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk 
      National University, Jeonju, Jeonbuk 561-156, Republic of Korea.
FAU - Kim, Sokho
AU  - Kim S
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk 
      National University, Jeonju, Jeonbuk 561-156, Republic of Korea.
FAU - Kwon, Jungkee
AU  - Kwon J
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk 
      National University, Jeonju, Jeonbuk 561-156, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141031
PL  - England
TA  - J Sci Food Agric
JT  - Journal of the science of food and agriculture
JID - 0376334
RN  - 0 (Nerve Growth Factors)
RN  - 3K9958V90M (Ethanol)
RN  - 5G06TVY3R7 (Rutin)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Ethanol/*pharmacology
MH  - Hippocampus/*cytology
MH  - Mice
MH  - Nerve Growth Factors/genetics/*metabolism
MH  - Neurons/*drug effects/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Rutin/*pharmacology
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - ethanol
OT  - neurodegenerative disease
OT  - neurotrophic factors
OT  - oxidative stress
OT  - rutin
EDAT- 2014/09/25 06:00
MHDA- 2016/06/03 06:00
CRDT- 2014/09/25 06:00
PHST- 2014/07/10 00:00 [received]
PHST- 2014/09/17 00:00 [revised]
PHST- 2014/09/18 00:00 [accepted]
PHST- 2014/09/25 06:00 [entrez]
PHST- 2014/09/25 06:00 [pubmed]
PHST- 2016/06/03 06:00 [medline]
AID - 10.1002/jsfa.6927 [doi]
PST - ppublish
SO  - J Sci Food Agric. 2015 Aug 15;95(10):2117-23. doi: 10.1002/jsfa.6927. Epub 2014 
      Oct 31.