PMID- 25255222
OWN - NLM
STAT- MEDLINE
DCOM- 20150622
LR  - 20220408
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 9
DP  - 2014
TI  - Maternal LPS exposure during pregnancy impairs testicular development, 
      steroidogenesis and spermatogenesis in male offspring.
PG  - e106786
LID - 10.1371/journal.pone.0106786 [doi]
LID - e106786
AB  - Lipopolysaccharide (LPS) is associated with adverse developmental outcomes 
      including embryonic resorption, fetal death, congenital teratogenesis and fetal 
      growth retardation. Here, we explored the effects of maternal LPS exposure during 
      pregnancy on testicular development, steroidogenesis and spermatogenesis in male 
      offspring. The pregnant mice were intraperitoneally injected with LPS (50 microg/kg) 
      daily from gestational day (GD) 13 to GD 17. At fetal period, a significant 
      decrease in body weight and abnormal Leydig cell aggregations were observed in 
      males whose mothers were exposed to LPS during pregnancy. At postnatal day (PND) 
      26, anogenital distance (AGD), a sensitive index of altered androgen action, was 
      markedly reduced in male pups whose mothers were exposed to LPS daily from GD13 
      to GD 17. At PND35, the weight of testes, prostates and seminal vesicles, and 
      serum testosterone (T) level were significantly decreased in LPS-treated male 
      pups. At adulthood, the number of sperm was significantly decreased in male 
      offspring whose mothers were exposed to LPS on GD 13-17. Maternal LPS exposure 
      during gestation obviously diminished the percent of seminiferous tubules in 
      stages I-VI, increased the percent of seminiferous tubules in stages IX-XII, and 
      caused massive sloughing of germ cells in seminiferous tubules in mouse testes. 
      Moreover, maternal LPS exposure significantly reduced serum T level in male mice 
      whose mothers were exposed to LPS challenge during pregnancy. Taken together, 
      these results suggest that maternal LPS exposure during pregnancy disrupts T 
      production. The decreased T synthesis might be associated with LPS-induced 
      impairments for spermatogenesis in male offspring.
FAU - Wang, Hua
AU  - Wang H
AD  - Department of Toxicology, School of Public Health, Anhui Medical University, 
      Hefei, Anhui, China; Anhui Provincial Key Laboratory of Population Health & 
      Aristogenics, Hefei, Anhui, China.
FAU - Yang, Lu-Lu
AU  - Yang LL
AD  - Department of Toxicology, School of Public Health, Anhui Medical University, 
      Hefei, Anhui, China.
FAU - Hu, Yong-Fang
AU  - Hu YF
AD  - Department of Toxicology, School of Public Health, Anhui Medical University, 
      Hefei, Anhui, China.
FAU - Wang, Bi-Wei
AU  - Wang BW
AD  - Department of Toxicology, School of Public Health, Anhui Medical University, 
      Hefei, Anhui, China.
FAU - Huang, Yin-Yin
AU  - Huang YY
AD  - Department of Toxicology, School of Public Health, Anhui Medical University, 
      Hefei, Anhui, China.
FAU - Zhang, Cheng
AU  - Zhang C
AD  - Department of Toxicology, School of Public Health, Anhui Medical University, 
      Hefei, Anhui, China; Anhui Provincial Key Laboratory of Population Health & 
      Aristogenics, Hefei, Anhui, China.
FAU - Chen, Yuan-Hua
AU  - Chen YH
AD  - Department of Toxicology, School of Public Health, Anhui Medical University, 
      Hefei, Anhui, China; Anhui Provincial Key Laboratory of Population Health & 
      Aristogenics, Hefei, Anhui, China; Department of Histology and Embryology, Anhui 
      Medical University, Hefei, Anhui, China.
FAU - Xu, De-Xiang
AU  - Xu DX
AD  - Department of Toxicology, School of Public Health, Anhui Medical University, 
      Hefei, Anhui, China; Anhui Provincial Key Laboratory of Population Health & 
      Aristogenics, Hefei, Anhui, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140925
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Steroids)
RN  - 3XMK78S47O (Testosterone)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Animals
MH  - Body Weight/drug effects
MH  - Female
MH  - Fetus/drug effects
MH  - Leydig Cells/drug effects
MH  - Lipopolysaccharides/*adverse effects
MH  - Luteinizing Hormone/blood
MH  - Male
MH  - Maternal Exposure/*adverse effects
MH  - Mice
MH  - Organ Size/drug effects
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/*chemically induced/pathology
MH  - Prostate/drug effects/growth & development
MH  - Seminal Vesicles/drug effects/growth & development
MH  - Sperm Count
MH  - Spermatogenesis/*drug effects
MH  - Spermatozoa/cytology/drug effects
MH  - Steroids/*biosynthesis/blood
MH  - Testis/*drug effects/*growth & development
MH  - Testosterone/biosynthesis/blood
PMC - PMC4177809
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/09/26 06:00
MHDA- 2015/06/24 06:00
PMCR- 2014/09/25
CRDT- 2014/09/26 06:00
PHST- 2014/06/24 00:00 [received]
PHST- 2014/07/31 00:00 [accepted]
PHST- 2014/09/26 06:00 [entrez]
PHST- 2014/09/26 06:00 [pubmed]
PHST- 2015/06/24 06:00 [medline]
PHST- 2014/09/25 00:00 [pmc-release]
AID - PONE-D-14-27818 [pii]
AID - 10.1371/journal.pone.0106786 [doi]
PST - epublish
SO  - PLoS One. 2014 Sep 25;9(9):e106786. doi: 10.1371/journal.pone.0106786. 
      eCollection 2014.