PMID- 25255560
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 32
IP  - 7
DP  - 2014 Jul
TI  - [Rapid simultaneous determination of 53 beta-lactam antibiotics and their 
      metabolites in milk by ultra-performance liquid chromatography coupled with 
      triple quadrupole mass spectrometry].
PG  - 693-701
AB  - A rapid method for the determination of 53 beta-lactams and their metabolites 
      residues in milk was developed by ultra-performance liquid chromatography-triple 
      quadrupole mass spectrometry (UPLC-MS/MS). The method was based on protein 
      precipitation by adding equal quantity of acetonitrile, followed by the ultra 
      filtration of the extracts. The analysis of the residues was achieved on an 
      ACQUITY BEH C18 column with gradient elution using mobile phases of 0.1% formic 
      acid in water and acetonitrile. The analytes were detected by positive 
      electrospray ionization tandem mass spectrometry in the multiple reaction 
      monitoring (MRM) mode, and quantified by matrix-matched internal standard 
      calibration. The cycle time of each analysis was 10 min. Under the optimum 
      operation conditions, excellent linear dynamic range was observed from the 
      quantification limits to 200 microg/kg with the correlation coefficients better 
      than 0.991 1 for all the compounds. The average recoveries for all the 
      beta-lactams and their metabolites ranged from 71% to 121% with the relative 
      standard deviations of 1.7%-19% (n = 6). This method enabled the screening for 
      more than 50 samples per day by one person and also showed good performance for 
      quantitation, and allowed the determination of the beta-lactams and their 
      metabolites residues in milk below the maximum residue levels (MRLs) according to 
      the Bulletin No. 235 (2002) of Ministry of Agriculture, P. R. China and EU 
      Regulation 2377/90/EC (2008 Revised).
FAU - Zhang, Xiuyao
AU  - Zhang X
FAU - Cai, Xinxin
AU  - Cai X
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (beta-Lactams)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Drug Residues/*analysis
MH  - Food Contamination/*analysis
MH  - Milk/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
MH  - beta-Lactams/*analysis
EDAT- 2014/09/27 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/09/27 06:00
PHST- 2014/09/27 06:00 [entrez]
PHST- 2014/09/27 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2014.03019 [doi]
PST - ppublish
SO  - Se Pu. 2014 Jul;32(7):693-701. doi: 10.3724/sp.j.1123.2014.03019.