PMID- 25256490
OWN - NLM
STAT- MEDLINE
DCOM- 20151019
LR  - 20140926
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 113
DP  - 2014 Nov 26
TI  - Ultrasound-assisted enzymatic extraction and antioxidant activity of 
      polysaccharides from pumpkin (Cucurbita moschata).
PG  - 314-24
LID - S0144-8617(14)00702-4 [pii]
LID - 10.1016/j.carbpol.2014.07.025 [doi]
AB  - An efficient ultrasound-assisted enzymatic extraction (UAEE) of Cucurbita 
      moschata polysaccharides (CMCP) was established and the CMCP antioxidant 
      activities were studied. The UAEE operating parameters (extraction temperature, 
      ultrasonic power, pH, and liquid-to-material ratio) were optimized using the 
      central composite design (CCD) and the mass transfer kinetic study in UAEE 
      procedure was used to select the optimal extraction time. Enzymolysis and 
      ultrasonication that were simultaneously conducted was selected as the UAEE 
      synergistic model and the optimum extraction conditions with a maximum 
      polysaccharide yield of 4.33 +/- 0.15% were as follows: extraction temperature, 
      51.5  degrees C; ultrasonic power, 440 W; pH, 5.0; liquid-to-material ratio, 5.70:1 mL/g; 
      and extraction time, 20 min. Evaluation of the antioxidant activity in vitro 
      suggested that CMCP has good potential as a natural antioxidant used in the food 
      or medicine industry because of their high reducing power and positive radical 
      scavenging activity for DPPH radical.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Wu, Hao
AU  - Wu H
AD  - College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 
      266109, PR China.
FAU - Zhu, Junxiang
AU  - Zhu J
AD  - College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 
      266109, PR China.
FAU - Diao, Wenchao
AU  - Diao W
AD  - College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 
      266109, PR China.
FAU - Wang, Chengrong
AU  - Wang C
AD  - College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 
      266109, PR China. Electronic address: qauwcr@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140721
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - 0 (Antioxidants)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Antioxidants/*isolation & purification/*pharmacology
MH  - Cucurbita/*enzymology
MH  - Free Radical Scavengers/isolation & purification/pharmacology
MH  - Polysaccharides/*isolation & purification/*pharmacology
MH  - *Sound
OTO - NOTNLM
OT  - Antioxidant activity
OT  - Cucurbita moschata
OT  - Polysaccharide
OT  - Response surface methodology
OT  - Ultrasound-assisted enzymatic extraction
EDAT- 2014/09/27 06:00
MHDA- 2015/10/20 06:00
CRDT- 2014/09/27 06:00
PHST- 2014/02/03 00:00 [received]
PHST- 2014/07/11 00:00 [revised]
PHST- 2014/07/13 00:00 [accepted]
PHST- 2014/09/27 06:00 [entrez]
PHST- 2014/09/27 06:00 [pubmed]
PHST- 2015/10/20 06:00 [medline]
AID - S0144-8617(14)00702-4 [pii]
AID - 10.1016/j.carbpol.2014.07.025 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2014 Nov 26;113:314-24. doi: 10.1016/j.carbpol.2014.07.025. Epub 
      2014 Jul 21.