PMID- 25257453
OWN - NLM
STAT- MEDLINE
DCOM- 20150528
LR  - 20181202
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 49
IP  - 7
DP  - 2014 Jul
TI  - [Study of peroxisome proliferator-activated receptor-gamma coactivator-1alpha expression 
      and cytoapoptosis in masseter muscles of unilateral chewing rat].
PG  - 408-11
AB  - OBJECTIVE: To investigate the changes of peroxisome proliferator- activated 
      receptor-gamma coactivator -1alpha (PGC-1alpha) mRNA and cytoapoptosis in the rats' masseter 
      muscle which had been influenced by unilateral chewing, and to explore the 
      theoretical foundation of changes in masticatory muscles induced by unilateral 
      chewing. METHODS: The animal models were established by extracting the Wistar 
      rats' left maxillary molars. Thirty- six female Wistar rats were randomly divided 
      into four groups of 2, 4, 6 and 8 weeks, nine each. In each group there were six 
      rats with molar extracted and three as control. The Ca(2)(+) level was detected by 
      atomic spectrophotometric method. The relative expression of PGC-1alpha mRNA was 
      detected by real- time fluorescent quantitative PCR. The apoptosis index was 
      detected by Hoechst staining. RESULTS: The Ca(2)(+) level in the muscle on the 
      extraction side were significantly higher than that in the controls in the 
      beginning stage of unilateral chewing, and reached the peak at the 4th week 
      [(43.62 +/- 2.36) microg/g]. The relative expressions of PGC-1alpha increased from the 
      beginning and reached the maximum level at the 4th week [extraction side: (1.57 +/- 
      0.10); non-extraction side: (1.92 +/- 0.06)], while the relative expressions of 
      PGC-1alpha in 6 and 8 weeks decreased gradually [extraction side: (1.06 +/- 0.08), 
      (1.08 +/- 0.07); non- extraction side: (1.09 +/- 0.10), (1.11 +/- 0.08)]. The changes 
      of apoptosis index on non- extraction side increased continually and peaked at 
      the 6th week [(38.56 +/- 1.64)%]. CONCLUSIONS: PGC-1alpha and cytoapoptosis played 
      important roles in different stages of tissue remodeling induced by unilateral 
      chewing.
FAU - Yang, Yingying
AU  - Yang Y
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
FAU - Ding, Tingting
AU  - Ding T
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
FAU - Wu, Qingting
AU  - Wu Q
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
FAU - Kong, Jingjing
AU  - Kong J
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
FAU - Qi, Dong
AU  - Qi D
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China.
FAU - Ji, Ping
AU  - Ji P
AD  - Department of Prosthodontics, School of Stomatology, Shandong University; 
      Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China. 
      Email: jiping@mail.sdu.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Ppargc1a protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Female
MH  - Masseter Muscle/cytology/*metabolism
MH  - *Mastication
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
MH  - RNA, Messenger
MH  - RNA-Binding Proteins
MH  - Rats
MH  - Rats, Wistar
MH  - Transcription Factors/*metabolism
EDAT- 2014/09/27 06:00
MHDA- 2015/05/29 06:00
CRDT- 2014/09/27 06:00
PHST- 2014/09/27 06:00 [entrez]
PHST- 2014/09/27 06:00 [pubmed]
PHST- 2015/05/29 06:00 [medline]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2014 Jul;49(7):408-11.