PMID- 25257837
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160519
LR  - 20211021
IS  - 1699-3055 (Electronic)
IS  - 1699-048X (Linking)
VI  - 17
IP  - 3
DP  - 2015 Mar
TI  - Profiling of differentially expressed microRNAs (miRNAs) during differentiation 
      of rat hepatic oval cells (HOCs) into hepatocellular carcinoma (HCC) cells.
PG  - 230-7
LID - 10.1007/s12094-014-1218-2 [doi]
AB  - OBJECTIVE: To explore the expression profile of miRNAs during differentiation of 
      rat hepatic oval cells (HOCs) into hepatocellular carcinoma cells (HCC). METHODS: 
      Proliferation of rat HOCs was induced by chemical carcinogen, 
      3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) in male rats. By using Percoll 
      density gradient centrifugation method, HOCs were isolated, followed by 
      continuous cultivation in vitro. The isolated HOCs were identified via Thy-1 and 
      C-kit detection under laser scanning confocal microscope. Total miRNA was then 
      extracted from HOCs during cell differentiation for microarray hybridization. 
      Differentially expressed miRNAs among the indicated time points were identified. 
      The target genes of identified miRNAs were predicted using PicTar, Target-Scan, 
      and miRanda; then the functions and pathways of the genes were enriched. Y 
      chromosome-specific polymerase chain reaction (PCR) technique was utilized to 
      trace the differentiation of the male HOCs in carcinogen-induced HCC of female 
      rats. RESULTS: It was shown that isolated HOCs expressed stem cells markers of 
      Thy-1 and C-kit in cytoplasm and membrane. Among 1,210 miRNAs identified, 22 were 
      differentially expressed (P < 0.05, fold change >/=2), including 19 up-regulated 
      and 3 down-regulated ones. The predicted target genes of these miRNAs were 
      enriched in several functions, including axon guidance, angiogenesis, 
      post-transcriptional protein modification, and small molecular metabolism. For 
      PCR-based SRY detection, HCC genomic DNA of female rats from the experimental 
      group displayed the same PCR product as that from normal male rat. CONCLUSION: 
      Differentially expressed miRNAs exerted important roles during the 
      differentiation process of HOCs to HCC.
FAU - Xu, R H
AU  - Xu RH
AD  - Department of Oncology Surgery, The Affiliated Hospital of Hainan Medical 
      College, Haikou, 570102, China.
FAU - Zheng, L Y
AU  - Zheng LY
FAU - He, D L
AU  - He DL
FAU - Meng, J
AU  - Meng J
FAU - Xia, L P
AU  - Xia LP
FAU - Hao, X B
AU  - Hao XB
FAU - Zhang, Z Z
AU  - Zhang ZZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
PT  - Retraction of Publication
DEP - 20140926
PL  - Italy
TA  - Clin Transl Oncol
JT  - Clinical & translational oncology : official publication of the Federation of 
      Spanish Oncology Societies and of the National Cancer Institute of Mexico
JID - 101247119
RIN - Xu RH, Zheng LY, He DL, Meng J, Xia LP, Hao XB, Zhang ZZ. Clin Transl Oncol. 2015 
      Nov;17(11):935. PMID: 26282921
EDAT- 2014/09/27 06:00
MHDA- 2014/09/27 06:01
CRDT- 2014/09/27 06:00
PHST- 2014/04/17 00:00 [received]
PHST- 2014/08/16 00:00 [accepted]
PHST- 2014/09/27 06:00 [entrez]
PHST- 2014/09/27 06:00 [pubmed]
PHST- 2014/09/27 06:01 [medline]
AID - 10.1007/s12094-014-1218-2 [doi]
PST - ppublish
SO  - Clin Transl Oncol. 2015 Mar;17(3):230-7. doi: 10.1007/s12094-014-1218-2. Epub 
      2014 Sep 26.