PMID- 25258321
OWN - NLM
STAT- MEDLINE
DCOM- 20150117
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 289
IP  - 45
DP  - 2014 Nov 7
TI  - Absent, small or homeotic 2-like protein (ASH2L) enhances the transcription of 
      the estrogen receptor alpha gene through GATA-binding protein 3 (GATA3).
PG  - 31373-81
LID - 10.1074/jbc.M114.579839 [doi]
AB  - ASH2L is a component of MLL complexes that confer H3K4 trimethylation. The ASH2L 
      gene is located at 8q11-12, which is often amplified in breast cancers. We found 
      that increased ASH2L expression, which can result from gene amplification, is 
      often correlated with increased ERalpha expression in both breast cancer cell lines 
      and primary breast cancers. Forced expression of ASH2L induced ERalpha expression in 
      mammary epithelial cells, whereas depletion of ASH2L suppressed ERalpha expression in 
      breast cancer cells. To understand the mechanism by which ASH2L regulates ERalpha 
      expression, we identified GATA3 as the binding protein of ASH2L. ASH2L was shown 
      to potentiate the transcriptional activity of GATA3. ASH2L was recruited to the 
      enhancer of the ERalpha gene through GATA3 to promote ERalpha transcription. This study 
      established that ASH2L enhances ERalpha expression as a coactivator of GATA3 in 
      breast cancers.
CI  - (c) 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
FAU - Qi, Jin
AU  - Qi J
AD  - From the Maternal and Child Hospital of Shaanxi Province, Xian, Shaanxi, China.
FAU - Huo, Lei
AU  - Huo L
AD  - the Division of Pathology and Laboratory Medicine, The University of Texas M.D. 
      Anderson Cancer Center, Houston, Texas 77030, and.
FAU - Zhu, Yiwei Tony
AU  - Zhu YT
AD  - the Department of Pathology, Feinberg School of Medicine, Northwestern 
      University, Chicago, Illinois 60611.
FAU - Zhu, Yi-Jun
AU  - Zhu YJ
AD  - the Department of Pathology, Feinberg School of Medicine, Northwestern 
      University, Chicago, Illinois 60611 y-zhu2@northwestern.edu.
LA  - eng
GR  - R01 CA088898/CA/NCI NIH HHS/United States
GR  - CA 88898/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140925
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (ASH2L protein, human)
RN  - 0 (Ash2l protein, mouse)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ESR1 protein, human)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (Histones)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Breast Neoplasms/metabolism
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - DNA-Binding Proteins/genetics/*physiology
MH  - Enhancer Elements, Genetic
MH  - Estrogen Receptor alpha/genetics/*metabolism
MH  - Female
MH  - GATA3 Transcription Factor/*metabolism
MH  - Gene Expression Regulation
MH  - *Gene Expression Regulation, Neoplastic
MH  - HEK293 Cells
MH  - Histones/metabolism
MH  - Humans
MH  - Mice
MH  - Nuclear Proteins/genetics/*physiology
MH  - Signal Transduction
MH  - Transcription Factors/genetics/*physiology
MH  - Transcription, Genetic
MH  - Two-Hybrid System Techniques
PMC - PMC4223337
OTO - NOTNLM
OT  - Breast Cancer
OT  - Estrogen Receptor
OT  - GATA Transcription Factor
OT  - Gene Regulation
OT  - Oncogene
EDAT- 2014/09/27 06:00
MHDA- 2015/01/18 06:00
PMCR- 2015/11/07
CRDT- 2014/09/27 06:00
PHST- 2014/09/27 06:00 [entrez]
PHST- 2014/09/27 06:00 [pubmed]
PHST- 2015/01/18 06:00 [medline]
PHST- 2015/11/07 00:00 [pmc-release]
AID - S0021-9258(20)33364-0 [pii]
AID - M114.579839 [pii]
AID - 10.1074/jbc.M114.579839 [doi]
PST - ppublish
SO  - J Biol Chem. 2014 Nov 7;289(45):31373-81. doi: 10.1074/jbc.M114.579839. Epub 2014 
      Sep 25.